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中华诊断学电子杂志 ›› 2016, Vol. 04 ›› Issue (03) : 198 -202. doi: 10.3877/cma.j.issn.2095-655X.2016.03.013

所属专题: 文献

临床研究

谷胱甘肽-S转移酶基因多态性对6月龄幼儿智力发育的判断价值
贾晓1, 张倩1, 孙忠清2, 谢萌3, 王炳玲2,(), 逄淑涛4   
  1. 1. 266400 青岛市黄岛区妇幼保健与计划生育服务中心儿童保健科
    2. 266033 青岛市疾病预防控制中心环境食品卫生科
    3. 266021 青岛大学公共卫生学院流行病与卫生统计教研室
    4. 266071 青岛市中心血站
  • 收稿日期:2016-07-21 出版日期:2016-08-26
  • 通信作者: 王炳玲
  • 基金资助:
    国家自然科学基金(81372955)

Influence of the polymorphism of glutathione S-transferases on the intelligence development of six-month-old infants

Xiao Jia1, Qian Zhang1, Zhongqing Sun2, Meng Xie3, Bingling Wang2,(), Shutao Pang4   

  1. 1. Department of Child Care, Huangdao Maternal and Child Health Hospital of Qingdo, Qingdao 266400, China
    2. Department of Food and Environmental Health, Qingdao Centers for Disease Control and Prevention, Qingdao 266033, China
    3. Department of Epidemiology and Health Statistics, School of Public Health of Qingdao University, Qingdao 266021, China
    4. Qingdao Blood Center, Qingdao 266071, China
  • Received:2016-07-21 Published:2016-08-26
  • Corresponding author: Bingling Wang
  • About author:
    Corresponding author: Wang Bingling, Email:
引用本文:

贾晓, 张倩, 孙忠清, 谢萌, 王炳玲, 逄淑涛. 谷胱甘肽-S转移酶基因多态性对6月龄幼儿智力发育的判断价值[J]. 中华诊断学电子杂志, 2016, 04(03): 198-202.

Xiao Jia, Qian Zhang, Zhongqing Sun, Meng Xie, Bingling Wang, Shutao Pang. Influence of the polymorphism of glutathione S-transferases on the intelligence development of six-month-old infants[J]. Chinese Journal of Diagnostics(Electronic Edition), 2016, 04(03): 198-202.

目的

探讨幼儿谷胱甘肽-S转移酶(GST)基因多态性对6月龄幼儿智力发育的判断价值。

方法

利用559名幼儿分娩时的脐带血提取DNA,采用多重扩增技术测定谷胱甘肽-S-转移酶M1-02(GSTM1-02)的基因型,SequenomMassArray系统测定GSTM3(rs7483)和GSTT2(rs140195、rs2719)的基因型。使用盖泽尔发育诊断量表测定6月龄幼儿5个能区的发育情况。

结果

幼儿5个能区的发育商<90的占2.3%~6.8%。GSTM1-02的两种基因型分布相近,rs7483次要等位基因频率C=25.3%,rs140195次要等位基因频率A=35.2%,rs2719的次要等位基因频率T=25.6%。多因素分析显示rs7483的CC纯合子表现出了与幼儿适应性行为、大运动行为和精细动作行为得分相关性,OR及其95% CI分别为10.51(1.31,84.41)、4.44(1.18,16.72)和3.61(1.02,12.74)。

结论

GSTM3的rs7483位点多态性可能在6月龄时即可影响到幼儿的智力发育。

Objective

To explore the possible association between the polymorphism of glutathione S-transferases (GST) and the intelligence development of six-month-old infants.

Methods

DNA was extracted from the cord blood, and the GSTM1-02 genotypes were measured by multiple polymerase chain reaction (PCR), and the genotypes of GSTM3 (rs7483) and GSTT2(rs140195, rs2719) were measured by the SequenomMassArray system.Gesell development diagnostic scale was used to measure the development quotients (DQs) of 5 domains.

Results

The percentage of DQs less than 90 of the 5 domains was from 2.3% to 6.8%.The valid and invalid genotypes of GSTM1-02 was almost in the same percentage.The minor allele frequency of rs7483, rs140195 and rs2719 were 25.3%, 35.2% and 25.6%, respectively.Multivariate analysis showed that CC genotype indicated higher risk for lower DQs of adaptive, gross motor and fine motor behaviors compared to TT genotype with OR and 95% CI 10.51(1.31, 84.41), 4.44(1.18, 16.72)and 3.61(1.02, 12.74), respectively.

Conclusion

The polymorphism of rs7483 of GSTM3 may influence the intelligence development even when the infant is 6 months old.

表1 幼儿的一般情况
表2 幼儿男女发育商的分布情况
表3 幼儿GSTs酶不同基因型分布(n=559;例,%)
表4 单因素分析GSTs基因多态与幼儿智力发育的关联
表5 多因素logistic回归分析rs7483基因型与智力发育的关联
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