探讨脆弱气单胞菌感染性腹泻的实验诊断。

执行《全国临床检验操作规程》及《诊断细菌学》检验技术规范，应用细菌学鉴定的双岐索引技术，根据细菌的培养特性、细菌的表型生化反应特征，采用细菌微量生化反应管进一步做分离鉴定菌株的细菌学属/种的鉴定。细菌药敏试验采用纸片扩散法。

该菌株的细菌学培养特性，氧化发酵试验呈(F)型、氧化酶试验阳性，属于弧菌科细菌；O/129药敏试验示抗药，6%Nacl培养基中不生长，鉴定为气单胞菌属细菌。细菌微量生化反应试验结果，符合脆弱气单胞菌的细菌学描述。该菌株对氨苄西林和羧苄西林等敏感，仅对头孢噻吩耐药。

该病例细菌学检验诊断为脆弱气单胞菌感染性腹泻。气单胞菌生物学性状复杂、多变，应根据其多项关键性实验结果综合判断，且必须做好细菌检验的质量控制。

To explore the experimental diagnosis of infections diarrhea caused by aeromonas trota and literature review.

The operation performed the technical specification of "National Clinical Laboratory Procedures" and "Diagnostic Bacteriology" and applied bifidobacterium indexing technology.According to the cultural characteristics and phenotypic biochemical reactions characteristics of bacteria, of further isolation and identification of bacterial strains were done by using by micro-bacteria biochemical reaction tube.Bacterial susceptibility was tested by K-B method.

OF test was (F) type, oxidase test was positive, the bacteria belonged to Vibrio bacteria.O/129 susceptibility testing showed drug resistance, it didn′t grow in 6% Nacl medium which was identified as aeromonas bacteria.The result of bacterial micro biochemical reaction test was in line with descripton of aeromonas trota.The strains were sensitive to ampicillin and carbenicillin, only resistant to cephalothin.

The case is diagnosed as infectious diarrhea caused by aeromonas trota.The biological activity of aeromonas is complex and changeable, and should be estimated by a number of key experimental results.The quality of the bacteria detection should be controled.