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中华诊断学电子杂志 ›› 2017, Vol. 05 ›› Issue (03) : 196 -199. doi: 10.3877/cma.j.issn.2095-655X.2017.03.014

所属专题： 文献；

临床研究

Apelin和血管紧张素受体AT1相关受体蛋白在胃癌中表达及其临床意义
张慧1, 李建军2,†(), 李雷3, 赵丽华3, 王爱亮4, 冯其贞5   
  1. 1. 272067　济宁医学院基础医学院组织学与胚胎学教研室
    2. 272067　济宁医学院临床医学院
    3. 272029　济宁医学院附属医院病理科
    4. 272029　济宁医学院胃肠外科
    5. 272067　济宁医学院康复医学院
  • 收稿日期:2017-06-27 出版日期:2017-08-26
  • 通信作者: 李建军
  • 基金资助:
    山东省自然科学基金(ZR2013HL011)

The expression of apelin and putative receptor protein related to the angiotensin receptor AT1 in human gastric cancer and their clinicopathologic significance

Hui Zhang1, Jianjun Li2,†(), Lei Li3, Lihua Zhao3, Ailiang Wang4, Qizhen Feng5   

  1. 1. Department of Histology and Embryology, Jining Medical University, Jining 272067, China
    2. Clinical College, Jining Medical University, Jining 272067, China
    3. Department of Pathology, Affiliated Hospital of Jining Medical University, Jining 272029, China
    4. Department of Gastroenterological Surgery, Affiliated Hospital of Jining Medical University, Jining 272029, China
    5. School of Rehabilitation Medicine, Jining Medical University, Jining 272067, China
  • Received:2017-06-27 Published:2017-08-26
  • Corresponding author: Jianjun Li
  • About author:
    Corresponding author: Li Jianjun, Email:
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引用本文：

张慧, 李建军, 李雷, 赵丽华, 王爱亮, 冯其贞. Apelin和血管紧张素受体AT1相关受体蛋白在胃癌中表达及其临床意义[J]. 中华诊断学电子杂志, 2017, 05(03): 196-199.

Hui Zhang, Jianjun Li, Lei Li, Lihua Zhao, Ailiang Wang, Qizhen Feng. The expression of apelin and putative receptor protein related to the angiotensin receptor AT1 in human gastric cancer and their clinicopathologic significance[J]. Chinese Journal of Diagnostics(Electronic Edition), 2017, 05(03): 196-199.

目的

探讨胃癌组织中apelin和血管紧张素受体AT1相关受体蛋白(APJ)的表达及其临床意义。

方法

标本来源于2015年1月至12月济宁医学院附属医院胃肠外科收治的123例胃癌患者，自医院病理科调取上述患者的癌组织及正常黏膜组织的蜡块，采用SP免疫组织化学染色法对标本中apelin、APJ的表达水平并进行了半定量分析，通过计算CD34标记的微血管评价"热点"血管密度(hMVD)。

结果

Apelin和APJ在正常胃黏膜的腺细胞均呈阳性表达、但是在上皮层未见有表达；apelin主要表达于胃癌细胞的细胞质和细胞核，但未见APJ在胃癌细胞中的表达；CD34在肿瘤组织中微血管计数明显高于正常胃粘膜；Apelin的表达水平在不同的胃癌患者年龄、性别和TNM分期之间的差异无统计学意义，肿瘤的分化程度(3.16±2.10，3.55±2.01，4.36±2.18；F＝20.56，P<0.05)和微"热点"血管密度(3.35±2.14，4.23±2.05；z＝12.37，P<0.05)差异有统计学意义。

结论

Apelin蛋白参与了胃癌的发生发展，其表达水平与肿瘤分化程度、hMVD有关，在低分化、高hMVD的肿瘤组织中呈现较高表达水平。

关键词: Apelin, 血管紧张素受体AT1相关的受体蛋白, 抗原，CD34, 胃肿瘤
Objective

To study the expression level of apelin and angiotensin receptor AT1(APJ)in gastric cancer and explore possible association between the expression of Apelin/APJ and clinical significance.

Methods

Cancer tissues and normal mucosa tissues with wax block of 123 cases of gastric cancer patients, who received surgery of gastrointestinal surgery in Jining medical college affiliated hospital from January 2015 to December 2015 were collected from pathology department.SP immunohistochemical staining was used to explore the expression level of apelin and APJ.Semi-quantitative analysis was carried out.Through calculating microvasculars labelled by CD34 markers, " hot spots" microvessel densitic (hMVD) was calculated.

Results

Both apelin and APJ showed positive expression in cytoplasm of normal gastric mucosa, but no expression was found in the upper cortex.Apelin was mainly expressed in the cytoplasm and nucleus of gastric cancer cells, but no APJ was expressed in gastric cancer.CD34 was significantly higher in tumor tissue than that in normal gastric mucosa.There were no significant difference for apelin expressing levels in different age (3.56±2.13, 3.98±2.08), sex (3.62±2.21, 3.96±2.27) and TNM (3.66±2.36, 4.14±2.12) staging of gastric cancer patients.However, there were significant differences between different tumor differentiation degree (3.16±2.10, 3.55±2.01, 4.36±2.18; F=20.56, P<0.05) and high-microvessel densitie (hMVD)(3.35±2.14, 4.23±2.05; z=12.37, P<0.05).

Conclusions

Apelin is involved in the development of gastric cancer, and its expression level is related to the degree of tumor differentiation and hMVD.High expression level of apelin is presented in tumor tissues with low differentiation and high density hMVD.

Key words: Apelin, Putative receptor protein related to the angiotensin receptor AT1, Antigens, CD34, Stomach neoplasms
表1 胃癌患者的肿瘤临床病理学参数特点及apelin在胃癌中的表达情况(n＝123)
项目 例数 Apelin F值/z值 P值
年龄(岁) ? ? Z＝0.86 0.27
? <58 55 3.56±2.13 ? ?
? ≥58 68 3.98±2.08 ? ?
性别 ? ? Z＝0.19 0.43
? 男 83 3.62±2.21 ? ?
? 女 40 3.96±2.27 ? ?
TNM分期 ? ? Z＝0.50 0.24
? Ⅰ～Ⅱ 52 3.66±2.36 ? ?
? Ⅲ～Ⅳ 71 4.14±2.12 ? ?
淋巴结转移情况 ? ? Z＝0.22 0.16
? N0 48 3.49±2.20 ? ?
? N＋ 75 4.09±2.41 ? ?
分化程度 ? ? F＝20.56 0.04
? 高分化 27 3.16±2.10 ? ?
? 中分化 50 3.55±2.01 ? ?
? 低分化 46 4.36±2.18 ? ?
hMVD ? ? Z＝12.37 0.02
? 低密度 52 3.35±2.14 ? ?
? 高密度 71 4.23±2.05 ? ?
图1 Apelin、APJ和CD34蛋白在胃腺癌和正常胃黏膜中的表达情况。在正常胃黏膜的上皮层未见apelin表达(a图箭头所示)；在正常胃黏膜的腺体细胞胞质中apelin呈现阳性表达(b图箭头所示)；在胃癌组织的胞浆及胞核中均可见apelin的阳性表达(c图、d图箭头所示)；在正常胃黏膜的上皮层未见APJ蛋白表达(e图箭头所示)；在正常胃粘膜的腺体细胞胞质中APJ呈现阳性表达(f图箭头所示)；在胃癌组织的胞质和胞核均未见APJ蛋白表达(g图箭头所示)；胃癌组织中，CD34标记的血管内皮细胞的细胞核增大，血管内皮细胞的数量增加(h图箭头所示)
[1]
Kim YH,Liang H,Liu X, et al.AMPKalpha modulation in cancer progression：multilayer integrative analysis of the whole transcriptome in Asian gastric cancer[J]. Cancer Res, 2012, 72(10): 2512-2521.
[2]
邹文斌,李兆申. 中国胃癌发病率及死亡率研究进展[J]. 中国实用内科杂志, 2014, 34(4): 408-415.
[3]
Wang XQ,Terry PD,Yan H. Review of salt consumption and stomach cancer risk：epidemiological and biological evidence[J]. World J Gastroenterol, 2009, 15(18): 2204-2213.
[4]
张萍,聂红峰,孙武装. Apelin在肺部疾病中的研究进展[J]. 河北医科大学学报, 2012, 33(9): 1110-1113.
[5]
Tatemoto K,Hosoya M,Habata Y, et al.Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor[J]. Biochem Biophys Res Commun, 1998, 251(2): 471-476.
[6]
Domazet I,Holleran BJ,Richard A, et al.Characterization of angiotensin II molecular determinants involved in AT1 receptor functional selectivity[J]. Mol Pharmacol, 2015, 87(6): 982-995.
[7]
Medhurst AD,Jennings CA,Robbins MJ, et al.Pharmacological and immunohistochemical characterization of the APJ receptor and its endogenous ligand apelin[J]. J Neurochem, 2003, 84(5): 1162-1172.
[8]
Machura E,Ziora K,Ziora D, et al.Serum apelin-12 level is elevated in schoolchildren with atopic asthma[J]. Respir Med, 2013, 107(2): 196-201.
[9]
朱智明,曹芳英. Apelin/APJ系统的生物特性及其与疾病的关系[J]. 天津医药, 2013, 41(2): 184187.
[10]
Yang Y,Lv SY,Ye W, et al.Apelin/APJ system and cancer[J]. Clin Chim Acta, 2016(457): 112-116.
[11]
Feng M,Yao G,Yu H, et al. Tumor apelin, not serum apelin, is associated with the clinical features and prognosis of gastric cancer[J]. BMC Cancer, 2016, 16(1): 794.
[12]
Wang G,Anini Y,Wei W, et al. Apelin, a new enteric peptide：localization in the gastrointestinal tract, ontogeny, and stimulation of gastric cell proliferation and of cholecystokinin secretion[J]. Endocrinology, 2004, 145(3): 1342-1348.
[13]
Chapman NA,Dupré DJ,Rainey JK.The apelin receptor：physiology, pathology, cell signalling, and ligand modulation of a peptide-activated class A GPCR[J]. Biochem Cell Biol, 2014, 92(6): 431-440.
[14]
Momiyama Y. Association between plasma apelin levels and coronary collateral development in patients with stable angina pectoris[J]. Atherosclerosis, 2014, 235(2): 349-350.
[15]
Ishida J,Hashimoto T,Hashimoto Y, et al.Regulatory roles for APJ, a seven-transmembrane receptor related to angiotensin-type 1 receptor in blood pressure in vivo[J]. J Biol Chem, 2004, 279(25): 26274-26279.
[16]
Muto J,Shirabe K,Yoshizumi T, et al. The apelin-APJ system induces tumor arteriogenesis in hepatocellular carcinoma[J]. Anticancer Res, 2014, 34(10): 5313-5320.
[17]
Lv X,Kong J,Chen WD, et al.The Role of the Apelin/APJ System in the Regulation of Liver Disease[J]. Front Pharmacol, 2017, 24(8): 221.
[18]
Maden M,Pamuk ON,Pamuk GE.High apelin levels could be used as a diagnostic marker in multiple myeloma：A comparative study[J]. Cancer Biomark, 2016, 17(4): 391-396.
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