[1] |
Taylor SC, Laperriere G, Germain H.Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets:from variable nonsense to publication quality data [J].Sci Rep,2017,7(1):2409.
|
[2] |
Lau BT, Wood-Bouwens C, Ji HP.Robust multiplexed clustering and denoising of digital PCR assays by data gridding[J].Anal Chem,2017,89(22):11913-11917.
|
[3] |
Pickar-Oliver A, Gersbach CA.The next generation of CRISPR-Cas technologies and applications[J].Nat Rev Mol Cell Biol,2019,20(8):490-507.
|
[4] |
Grissa I, Vergnaud G, Pourcel C,et al.The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats [J].BMC Bioinformatics,2007(8):172.
|
[5] |
Ishino Y, Shinagawa H, Makino K,et al.Nucleotide sequence of the iap gene,responsible for alkaline phosphatase isozyme conversion in Escherichia coli,and identification of the gene product[J].J Bacteriol,1987,169(12):5429-5433.
|
[6] |
Makarova KS, Wolf YI, Alkhnbashi OS,et al.An updated evolutionary classification of CRISPR-Cas systems [J].Nat Rev Microbiol,2015,13(11):722-736.
|
[7] |
Makarova KS, Wolf YI, Koonin EV.The basic building blocks and evolution of CRISPR-Cas systems [J].Biochem Soc Trans,2013,41(6):1392-1400.
|
[8] |
Rouillon C, Zhou M, Zhang J, et al. Structure of the CRISPR interference complex CSM reveals key similarities with Cascade [J].Mol Cell,2013,52(1):124-134.
|
[9] |
Shmakov S, Abudayyeh OO, Makarova KS,et al.Discovery and functional characterization of diverse class 2 CRISPR-Cas systems [J].Mol Cell,2015,60(3):385-397.
|
[10] |
Hu JH, Davis KM, Liu DR.Chemical biology approaches to genome editing:understanding,controlling,and delivering programmable nucleases [J].Cell Chem Biol,2016,23(1):57-73.
|
[11] |
Zetsche B, Gootenberg JS, Abudayyeh OO,et al.Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system [J].Cell,2015,163(3):759-771.
|
[12] |
Gao P, Yang H, Rajashankar KR,et al.Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition [J].Cell Res,2016,26(8):901-913.
|
[13] |
Li SY, Cheng QX, Liu JK,et al.CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA [J].Cell Res,2018,28(4):491-493.
|
[14] |
Abudayyeh OO, Gootenberg JS, Konermann S, et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector [J].Science,2016,353(6299):5573.
|
[15] |
Jinek M, Chylinski K, Fonfara I,et al.A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity [J].Science,2012,337(6096):816-821.
|
[16] |
Strutt SC, Torrez RM, Kaya E, et al. RNA-dependent RNA targeting by CRISPR-Cas9 [J].Elife,2018(7):e32724.
|
[17] |
Pardee K, Green AA, Takahashi MK, et al. Rapid, low-cost detection of Zika virus using programmable biomolecular components[J].Cell,2016,165(5):1255-1266.
|
[18] |
Muller V, Rajer F, Frykholm K, et al. Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping [J].Sci Rep,2016 (6):37938.
|
[19] |
Quan J, Langelier C, Kuchta A,et al.FLASH:a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences[J].Nucleic Acids Res,2019,47(14):e83.
|
[20] |
Chen JS, Ma E, Harrington LB, et al. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity [J].Science,2018,360(6387):436-439.
|
[21] |
Li SY, Cheng QX, Wang JM,et al.CRISPR-Cas12a-assisted nucleic acid detection [J].Cell Discov,2018 (4):20.
|
[22] |
He Q, Yu D, Bao M,et al.High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system [J].Biosens Bioelectron,2020(154):112068.
|
[23] |
Tao D, Liu J, Nie X, et al. Application of CRISPR-Cas12a enhanced fluorescence assay coupled with nucleic acid amplification for the sensitive detection of African Swine Fever Virus[J].ACS Synth Biol,2020,9(9):2339-2350.
|
[24] |
Bai J, Lin H, Li H,et al.Cas12a-based on-site and rapid nucleic acid detection of African Swine Fever[J].Front Microbiol,2019(10):2830.
|
[25] |
杨策.新型冠状病毒肺炎诊治——殊死搏斗中的困境与挑战[J/CD].中华诊断学电子杂志,2020,8(1):1-8.
|
[26] |
Broughton JP, Deng X, Yu G, et al. CRISPR-Cas12-based detection of SARS-CoV-2 [J].Nat Biotechnol,2020,38(7):870-874.
|
[27] |
Ding X, Yin K, Li Z, et al.All-In-One Dual CRISPR-Cas12a (AIOD-CRISPR) assay:a case for rapid,ultrasensitive and visual detection of novel Coronavirus SARS-CoV-2 and HIV virus[J]. Nat Commun,2020,11(1):4711.
|
[28] |
Ali Z, Aman R, Mahas A,et al.iSCAN:an RT-LAMP-coupled CRISPR-Cas12 module for rapid,sensitive detection of SARS-CoV-2 [J].Virus Res,2020 (288):198129.
|
[29] |
Ai JW, Zhou X, Xu T, et al. CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis [J].Emerg Microbes Infect,2019,8(1):1361-1369.
|
[30] |
East-Seletsky A, O'Connell MR, Knight SC,et al.Two distinct RNase activitiesof CRISPR-C2c2 enable guide-RNA processing and RNA detection[J].Nature,2016,538(7624):270-273.
|
[31] |
Gootenberg JS, Abudayyeh OO, Lee JW, et al. Nucleic acid detection with CRISPR-Cas13a/C2c2[J].Science,2017,356(6336):438-442.
|
[32] |
Myhrvold C, Freije CA, Gootenberg JS,et al.Field-deployable viral diagnostics using CRISPR-Cas13 [J].Science,2018,360(6387):444-448.
|
[33] |
Wu Y, Liu SX, Wang F, et al. Room temperature detection of plasma Epstein-Barr virus DNA with CRISPR-Cas13 [J].Clin Chem,2019,65(4):591-592.
|
[34] |
Qin P, Park M, Alfson KJ,et al.Rapid and fully microfluidic Ebola virus detection with CRISPR-Cas13a [J].ACS Sens,2019,4(4):1048-1054.
|
[35] |
Barnes KG, Lachenauer AE, Nitido A, et al.Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time[J].Nat Commun,2020,11(1):4131.
|
[36] |
Wang S, Li H, Kou Z,et al.Highly sensitive and specific detection of hepatitis B virus DNA and drug resistance mutations utilizing the PCR-based CRISPR-Cas13a system[J/OL].Clin Microbiol Infect,2020.[2020-10-14].published online ahead of print April 29,2020].
URL
|
[37] |
Liu Y, Xu H, Liu C, et al.CRISPR-Cas13a nanomachine based simple technology for avian influenza A (H7N9) virus on-site detection[J].J Biomed Nanotechnol,2019,15(4):790-798.
|
[38] |
Arizti-Sanz J, Freije CA, Stanton AC, et al. Integrated sample inactivation,amplification,and Cas13-based detection of SARS-CoV-2 [J/OL].bioRxiv,2020.[2020-10-14].published online ahead of print May 28,2020].
URL
|
[39] |
Patchsung M, Jantarug K, Pattama A,et al.Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA[J/OL].Nat Biomed Eng,2020.[2020-10-14].published online ahead of print August 26,2020].
URL
|
[40] |
Ackerman CM, Myhrvold C, Thakku SG, et al.Massively multiplexed nucleic acid detection with Cas13 [J].Nature,2020,582(7811):277-282.
|
[41] |
Gootenberg JS, Abudayyeh OO, Kellner MJ,et al.Multiplexed and portable nucleic acid detection platform with Cas13,Cas12a,and Csm6 [J].Science,2018,360(6387):439-444.
|