探讨CD11b基因rs4597342位点多态性与川崎病(KD)及其冠状动脉损伤(CAL)的关系。

选择株洲市中心医院儿科2019年1月1日至2021年12月30日136例KD患儿(KD组)及同期74例健康儿童(对照组)。KD患儿根据有无CAL分为KD合并冠脉损伤组(KD-CAL组，n＝25)和无冠脉损伤组(KD-NCAL组，n＝111)。基于限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术结合Sanger测序法检测KD组患儿与对照组儿童的CD11b基因rs4597342位点多态性，χ²检验比较KD组和对照组，及KD-CAL与KD-NCAL组样本间rs4597342基因型及等位基因频率分布差异。

KD组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为5.89%、47.79%、46.32%，T、C等位基因频率分别为29.78%，70.22%；健康组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为1.35%、50.00%、48.65%，T、C等位基因频率分别为26.35%、73.65%。两组间基因型及等位基因频率分布比较，均差异无统计学意义(χ²＝2.40，0.55；均P>0.05)。KD-CAL组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为4.00%，48.00%，48.00%, T、C等位基因频率分别为28.00%，72.00%; KD-NCAL组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为6.31%，47.75%，45.94%；T、C等位基因频率分别为30.18%，69.82%，两组间比较，均差异无统计学意义(χ²＝0.20，0.09；均P>0.05)。

CD11b基因rs4597342位点多态性与KD及其CAL的发生无明显相关性。

To investigate the correlation between the rs4597342 polymorphism of the CD11b gene and Kawasaki disease(KD) and coronary artery lesions(CAL).

From January 1, 2019, to December 30, 2021, a total of 136 children with KD and 74 healthy children were selected from the Pediatrics Department of Zhuzhou Central Hospital. KD children were divided into KD with CAL group(KD-CAL group, n=25) and without CAL group (KD-NCAL group, n=111). The rs4597342 polymorphism of CD11b gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with Sanger sequencing method. The genotype and allele frequency distribution of rs4597342 between KD and control groups, as well as between the KD-CAL and KD-NCAL groups were compared by using the χ² test.

The TT, TC, and CC genotype distribution frequencies at rs4597342 of the CD11b gene in the KD group were 5.89%, 47.78%, and 46.32%, respectively, and the T and C allele frequencies were 29.78%, 70.22%, respectively. The TT, TC, CC genotype distribution frequencies at rs4597342 of the CD11b gene in the healthy group were 1.35%, 50.00%, 48.65%, respectively, and the T and C allele frequencies were 26.35%, 73.65%, respectively. There were no significant differences in genotype and allele frequency distribution between the two groups (χ²=2.40, 0.55, all P>0.05). The TT, TC, and CC genotype distribution frequencies at rs4597342 of CD11b gene in KD-CAL group were 4.00%, 48.00% and 48.00%, respectively, and the frequencies of T and C alleles were 28.00%, 72.00%, respectively. TT, TC, and CC genotype distribution frequencies at rs4597342 of CD11b gene in KD-NCAL group were 6.31%, 47.75% and 45.94%, respectively, and T and C allele frequencies were 30.18%, 69.82%, respectively. There were no significant differences between the two groups (χ²=0.20, 0.09, all P>0.05).

The rs4597342 polymorphism of CD11b gene and KD or its complication CAL are not significantly correlated.