Effect and significance of losartan on the angiotensin converting enzyme 2 in therosclerotic plaques

doi:10.3877/cma.j.issn.2095-655X.2014.02.013

Abstract

Abstract:

Objective

To investigate the effect of losartan on angiotensin converting enzyme 2 (ACE2)activity and ACE2、Ang-(1-7) protein level in rabbit atherosclerotic plaques and analyse its meaning.

Methods

Male New Zealand White rabbits were constructed atherosclerosis models.The rabbits were randomly 3 groups: high cholesterol group, losartan group and losartan+ A779 group.ACE2 activities were measured by enzymatic assay.The lipid content was evaluated by Oil red O stain, the proteins expression of ACE2、Ang-(1-7) and macrophage infiltration were also detected by immunohistochemistry.The differences of the protein expression of ACE2 were compared with chi-square test and t-test.

Results

The ACE2 activity higher in losartan group [(2.66±0.19)U/(μg protein·h)] and Losartan+ A779 group [(2.57±0.17)U/(μg protein·h)] than those in high-cholesterol group [(1.30±0.18)U/(μg protein·h)], and there was significant difference(t=15.87, 15.45; P<0.05). The lipid content and macrophage infiltration in losartian group[(22.92±2.4)% and (15.71±2.46)% respectly] were significantly lower than those of in high-cholesterol group[(30.47±1.83)% and (23.07±2.06)%] and in Losartan+ A779 group[(32.52±2.88)% and (22.91±2.11%)]. The expression of ACE2 and Angiotensin-(1-7) in losartan group[(0.2330±0.0291), (0.2652±0.0234)] and in losartan + A779 group[(0.2224±0.0168, 0.2583±0.0236)] were significant higher than that in high-cholesterol group[(0.1194±0.0114, 0.1580±0.01932)]. However, there was no difference between losartan group and losartan+ A779 group (P>0.05).

Conclusion

The results show that ACE2 activity and ACE2、Ang-(1-7) protein are regulated in atherosclerotic plaque by losartan, which may play an important role in the treatment of AS, proterin and ACE2, Ang-(1-7) protein.

Key words: Losartan, Angiotensins, Peptidyl-dipeptidase A, Atherosclerosis

Yuehui Zhang, Li Zhu, Yonghuan Zhang, Qingtao Yu, Zhaoli Zhou, Shuying Li, Xiaoyu Wang, Xuhong Zhang, Bo Dong. Effect and significance of losartan on the angiotensin converting enzyme 2 in therosclerotic plaques[J]. Chinese Journal of Diagnostics(Electronic Edition), 2014, 02(02): 121-126.

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References 17

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组别	动物数	0周		12周
组别	动物数	TC	LDL-C	TC	LDL-C
高脂组	9	0.82±0.21	0.51±0.14	35.85±7.21	13.87±1.14
氯沙坦组	9	0.83±0.22	0.52±0.11	35.32±7.53	13.66±1.08
氯沙坦＋A779组	9	0.82±0.20	0.50±0.13	35.67±7.86	13.79±1.27
F值	?	0.007	0.047	0.012	0.080
P值	?	0.993	0.955	0.988	0.924

组别	动物数	0周		12周
组别	动物数	TC	LDL-C	TC	LDL-C
高脂组	9	0.82±0.21	0.51±0.14	35.85±7.21	13.87±1.14
氯沙坦组	9	0.83±0.22	0.52±0.11	35.32±7.53	13.66±1.08
氯沙坦＋A779组	9	0.82±0.20	0.50±0.13	35.67±7.86	13.79±1.27
F值	?	0.007	0.047	0.012	0.080
P值	?	0.993	0.955	0.988	0.924

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