The experimental study of 5-fluorouracil magnetic albumin microspheres enhancing radiofrequency ablation in ex vivo bovine liver

doi:10.3877/cma.j.issn.2095-655X.2014.03.009

Abstract

Abstract:

Objective

To investigate the influence of radio-frequency ablation(RFA) combined with 5-Fu-MAMS injection on the ablation volume.

Methods

The subjects used in this experiment were fresh ex vivo bovine livers.Three groups were set up: Group Ⅰ, RFA alone; group Ⅱ, RFA combined with 5-fluorouracil; group Ⅲ, RFA combined with 5-fluorouracil magnetic albumin microspheres.The size and shape of coagulation necrosis zones were observed and measured.Single factor analysis of variance was used to analyze the affecting factors for the size of ablation lesions.Macroscopic and microscopic morphological characteristics of lesions were observed.

Results

In gross examination, all ablation lesions were gray elliptical in cross section and there were classical coagulation necrosis.The central organization was drying, and the treatment area and surrounding liver tissue boundaries were clear.The mean biggest longitudinal diameter and transverse diameter of necrosis volume of group I, group II and group III were (4.45±0.23)cm, (4.93±0.53)cm, (5.68±0.59)cm(F=10.23, P<0.05)and (2.83±0.25)cm, (3.08±0.22)cm, (4.10±0.14)cm respectively(F=61.23, P<0.05). The necrosis volumes of group Ⅲ(RFA combined with 5-Fu-MAMS) were bigger than those of control group(P<0.05). The mean durations were (472.00±20.49)s, (632.50±21.05)s and (836.50±19.10)s (F=489.28, P<0.05). The average temperature at 3 cm distance from the center of the puncture point were (41.83±2.04)℃, (47.33±3.83)℃ and (55.67±2.16)℃ (F=37.20, P<0.05). All the differences were statistically significant.

Conclusions

Compared with other two groups, RFA combined with 5-fluorouracil can produce larger ablation zones.The lesions typically showed coagulation necrosis at microscopicaI examination.

Key words: Catheter ablation, Fluorouracil, Albumins, Microspheres, Animals experimentation

Jiangang Liu, Seng Qiao, Changxiu Yuan, Zhichao Liu, Wen Cui, Guilin Chen. The experimental study of 5-fluorouracil magnetic albumin microspheres enhancing radiofrequency ablation in ex vivo bovine liver[J]. Chinese Journal of Diagnostics(Electronic Edition), 2014, 02(03): 202-205.

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组别	时间(s)	3 cm处温度(℃)	消融灶
组别	时间(s)	3 cm处温度(℃)	纵径(cm)	横径(cm)
A组	472.00±20.49	41.83±2.04	4.45±0.23	2.83±0.25
B组	632.50±21.05^a	47.33±3.83^a	4.93±0.53	3.08±0.22
C组	836.50±19.10^ab	55.67±2.16^ab	5.68±0.59^ab	4.10±0.14^ab
F值	489.28	37.20	10.14	62.43
P值	<0.05	<0.05	<0.05	<0.05

组别	时间(s)	3 cm处温度(℃)	消融灶
组别	时间(s)	3 cm处温度(℃)	纵径(cm)	横径(cm)
A组	472.00±20.49	41.83±2.04	4.45±0.23	2.83±0.25
B组	632.50±21.05^a	47.33±3.83^a	4.93±0.53	3.08±0.22
C组	836.50±19.10^ab	55.67±2.16^ab	5.68±0.59^ab	4.10±0.14^ab
F值	489.28	37.20	10.14	62.43
P值	<0.05	<0.05	<0.05	<0.05

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