Identification of corynebacterium jeikeium by detection of bacterial 16s ribosomal RNA gene

doi:10.3877/cma.j.issn.2095-655X.2015.04.019

Abstract

Abstract:

Objective

To detect clinical strain by amplifying 16S ribosomal RNA(16S rRNA) gene with polymerase chain reaction (PCR) method.

Methods

16S rRNA gene was amplifyied by universal primers P1, P2 with polymerase chain reaction, and the polymerase chain reaction products were sequenced to achieve the DNA sequences.

Results

PCR products were about 1 465bp, and the PCR sequences were blasted in NCBI web to achieve the proper strain name, with the use of this method, the unkown gram-positive bacilli strain was identified as corynebacterium jeikeium strain, ATCC25923 was identified as a strain of staphylococcus aureus and negative control was also specific.

Conclusion

16S rRNA gene detection can be used as strain identification, and especially for some less common clinical strains.

Key words: Bacteria, RNA, 16S, ribosomal, Polymerase chain reaction

Yingpeng Cui, Zhuliang Huang. Identification of corynebacterium jeikeium by detection of bacterial 16s ribosomal RNA gene[J]. Chinese Journal of Diagnostics(Electronic Edition), 2015, 03(04): 296-299.

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Figures/Tables 4

References 7

[1]	雷正瑜.16S rDNA序列分析技术在微生物分类鉴定做的应用. 湖北生态工程职业技术学院学报[J].2006, 4(1): 4-7.
[2]	杨霞,陈陆,王川庆.16S rRNA基因序列分析技术在细菌分类中应用的研究进展[J]. 西北农林科技大学学报(自然科学版), 2008, 36(2): 55-60.
[3]	卫俊杰,刘华,赵有成. 应用PCR方法检测重症合并感染病人血中细菌16SrRNA基因的临床价值[J]. 云南医药, 2008, 29(5): 447-449.
[4]	陈东科,孙长贵,魏莲花, 等. 实用临床微生物学检验与图谱[M].1版. 北京: 人民卫生出版社, 2011: 242-254.
[5]	胡晓红,彭惠民,刘昕, 等. PCR及Real-time PCR评价细菌DNA提取方法[J]. 重庆医科大学学报, 2008, 33(2): 155-158.
[6]	金中淦,葛平,徐蓉, 等.16S rRNA、16S-23S rRNA基因测序分析检测主要血流感染病原菌比较[J]. 分子诊断与治疗杂志, 2012, 4(3): 181-185.
[7]	鲁辛辛,黄艳飞,田晓波. 细菌rDNA分类鉴定的方法学研究[J]. 中华检验医学杂志, 2006, 29(5): 435-437.

引物	引物序列(5’-3’)	Tm(℃)	扩增片段bp	引物长度bp
Eubac27F(p1)	AGAGTTTGATCCTGGCTCAG	57.4	1 456	20
Eubac1492R(p2)	GGTTACCTTGTTACGACTT	52.4	1 456	19

引物	引物序列(5’-3’)	Tm(℃)	扩增片段bp	引物长度bp
Eubac27F(p1)	AGAGTTTGATCCTGGCTCAG	57.4	1 456	20
Eubac1492R(p2)	GGTTACCTTGTTACGACTT	52.4	1 456	19

试剂	体积(μL)
PCR buffer	5
Primer 1	1
Primer 2	1
dNTP	5
Tap DNA聚合酶	1.5
模板	5
去离子水	31.5

试剂	体积(μL)
PCR buffer	5
Primer 1	1
Primer 2	1
dNTP	5
Tap DNA聚合酶	1.5
模板	5
去离子水	31.5

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