Expression and significance of miRNA-23a in patients with dilated cardiomyopathy

doi:10.3877/cma.j.issn.2095-655X.2016.03.015

Abstract

Abstract:

Objective

To observe the expression of miR-23a in dilated cardiomyopathy (DCM) of the peripheral blood and assess its clinical significance.

Methods

Twenty-six patients with dilated cardiomyopathy and 30 healthy controls were enrolled.The clinical data of the two groups, cardiac ultrasound, plasma level of brain natriuretic peptide (BNP) and other biochemical indicators were collected respectively.Real-time polymerase chain reaction (PCR) was used to detect and compare two groups′s miR-23a expression level differences, ROC curve analysis was used to assess miR-23a recognition in patients with DCM.

Results

BNP (605.00±231.71, 26.29±17.88, t=218.61, P<0.05) and left ventricular end-diastdic diameter (LVEDD) (64.73±5.74, 45.58±2.17, t=6.09, P<0.05) in DCM group were higher than those in control group, left ventricular ejection fraction (LVEF) lower than that in control group (35.33±4.31, 58.72±3.35, t=-13.54, P<0.05). miR-23a expression in the DCM group was higher than that in control group (33.56±2.56, 1.18±0.86, t=2.85, P<0.01), the difference was statistically significant.ROC curve analysis demonstrated that miR-23a had certain clinical recognition ability in patients with DCM [AUC=0.781, 95% CI(0.651, 0.911), P<0.01].

Conclusion

The expression of miR-23a elevated in the peripheral blood of patients with DCM, miR-23a may have certain clinical diagnostic value in patients with DCM.

Key words: Cardiomyopathy, dilated, RNA, Polymerase chain reaction

Lixin Liu, Shaohui Zhang, Guanghe Wei, Yinfeng Jia, Ronghua Gao, Yuedong Lin, Zhencai Gao, Anyong Chen. Expression and significance of miRNA-23a in patients with dilated cardiomyopathy[J]. Chinese Journal of Diagnostics(Electronic Edition), 2016, 04(03): 206-209.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhzdxdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-655X.2016.03.015

https://zhzdxdzzz.cma-cmc.com.cn/EN/Y2016/V04/I03/206

Figures/Tables 2

References 21

[1]	Naga Prasad SV,Karnik SS.MicroRNAs-regulators of signaling networks in dilated cardiomyopathy.[J]. J Cardiovasc Transl Res, 2010, 3(3): 225-234.
[2]	Lin Z,Murtaza I,Wang K, et al.MiR-23a functions downstream of NFATc3 to regulate cardiac hypertrophy[J]. Proc Natl Acad Sci U S A, 2009, 106(29): 12103-12108.
[3]	中华医学会心血管病学分会, 中华心血管病杂志编辑委员会, 中国心肌病诊断与治疗建议工作组. 心肌病诊断与治疗建议[J]. 中华心血管病杂志, 2007, 35(1): 5-16.
[4]	Ha M,Kim VN.Regulation of microRNA biogenesis[J]. Nat Rev Mol Cell Biol, 2014, 15(8): 509-524.
[5]	Sayed D,Abdellatif M. MicroRNAs in development and disease[J]. Physiol Rev, 2011, 91(3): 827-887.
[6]	Condorelli G,Latronico MV,Cavarretta E. MicroRNAs in cardiovas cular diseases：current knowledge and the road ahead[J]. J Am Coll Cardiol, 2014, 63(21): 2177-2187.
[7]	Rottiers V,Näär AM. MicroRNAs in metabolism and metabolic disorders[J]. Nat Rev Mol Cell Biol, 2012, 13(4): 239-250.
[8]	Berindan-Neagoe I,Monroig Pdel C,Pasculli B, et al.Micro RNAome genome：a treasure for cancer diagnosis and therapy[J]. CA Cancer J Clin, 2014, 64(5): 311-336.
[9]	方喜生,刘国龙,刘霞. 微小RNA在肺腺癌发生与发展中的研究进展[J/CD]. 中华诊断学电子杂志, 2014, 2(3): 231-233.
[10]	Li J,Tan S,Kooger R, et al.MicroRNAs as novel biological targets for detection and regulation[J]. Chem Soc Rev, 2014, 43(2): 506-517.
[11]	Wang K,Lin ZQ,Long B, et al.Cardiac hypertrophy is positively regulated by microRNA miR-23a[J]. J Biol Chem, 2012, 287(1): 589-599.
[12]	Yang J,Nie Y,Wang F, et al.Reciprocal regulation of miR-23a and lysophosphatidic acid receptor signaling in cardiomyocyte hypertrophy[J]. Biochim Biophys Acta, 2013, 1831(8): 1386-1394.
[13]	Sun LY,Wang N,Ban T, et al.MicroRNA-23a mediates mitochondrial compromise in estrogen deficiency-induced concentric remodeling via targeting PGC-1α[J]. J Mol Cell Cardiol, 2014(75): 1-11.
[14]	Luo Z,Feng X,Wang H, et al.MiR-23a induces telomere dysfunction and cellular senescence by inhibiting TRF2 expression[J]. Aging Cell, 2015, 14(3): 391-399.
[15]	Nam S,Long X,Kwon C, et al.An integrative analysis of cellular contexts, miRNAs and mRNAs reveals network clusters associated with antiestrogen-resistant breast cancer cells[J]. BMC Genomics, 2012(13): 732.
[16]	Hausser J,Zavolan M. Identification and consequences of miRNA-target interactions-beyond repression of gene expression[J]. Nat Rev Genet, 2014, 15(9): 599-612.
[17]	赵雪,王玺,贺付成. 血清微小RNA-21在扩张型心肌病患者中的表达及其临床意义[J]. 中国全科医学, 2012, 15(36): 4190-4192.
[18]	Enes Coᶊkun M,Kervanciogˇglu M,öztuzcu S, et al.Plasma micro RNA profiling of children with idiopathic dilated cardiomyopathy[J]. Biomarkers, 2016, 21(1): 56-61.
[19]	Fan KL,Zhang HF,Shen J, et al.Circulating microRNAs levels in Chinese heart failure patients caused by dilated cardiomyopathy[J]. Indian Heart J, 2013, 65(1): 12-16.
[20]	Baumgarten A,Bang C,Tschirner A, et al.TWIST1 regulates the activity of ubiquitin proteasome system via the miR-199/214 cluster in human end-stage dilated cardiomyopathy[J]. Int J Cardiol, 2013, 168(2): 1447-1452.
[21]	Wijnen WJ,van der Made I,van den Oever S, et al.Cardiomyocyte-specific miRNA-30c over-expression causes dilated cardiomyopathy[J]. PLoS One, 2014, 9(5): e96290.

基线变量	DCM组(n＝26)	对照组(n＝30)	统计量	P值
年龄(岁)	43.21±4.82	42.18±4.59	t＝0.98	>0.05
男性(例，%)	19(73.08)	22(73.33)	χ²＝2.80	>0.05
BMI(kg/m²)	23.54±4.18	24.13±3.27	t＝－1.05	>0.05
心率(次/min)	70.55±11.09	71.35±9.84	t＝－1.08	>0.05
收缩压(mmHg)	115.47±11.46	116.26±10.29	t＝－0.06	>0.05
BNP(pg/ml)	605.00±231.71	26.29±17.88	t＝218.61	<0.05
LVEDD(mm)	64.73±5.74	45.58±2.17	t＝6.09	<0.05
LVEF(%)	35.33±4.31	58.72±3.35	t＝－13.54	<0.05

基线变量	DCM组(n＝26)	对照组(n＝30)	统计量	P值
年龄(岁)	43.21±4.82	42.18±4.59	t＝0.98	>0.05
男性(例，%)	19(73.08)	22(73.33)	χ²＝2.80	>0.05
BMI(kg/m²)	23.54±4.18	24.13±3.27	t＝－1.05	>0.05
心率(次/min)	70.55±11.09	71.35±9.84	t＝－1.08	>0.05
收缩压(mmHg)	115.47±11.46	116.26±10.29	t＝－0.06	>0.05
BNP(pg/ml)	605.00±231.71	26.29±17.88	t＝218.61	<0.05
LVEDD(mm)	64.73±5.74	45.58±2.17	t＝6.09	<0.05
LVEF(%)	35.33±4.31	58.72±3.35	t＝－13.54	<0.05

Please choose a citation manager

Content to export