Massive open online course (MOOC) as a new type of online open courses has been widely used in the field of education for its advantages of better interaction, fragmentation and holistic of learning.MOOC is often designed to the forms of micro-video, including offline and online courses.The application of MOOC in diagnostics teaching can cover most of the knowledge points with the advantage of network resources, consider various levels of medical students with autonomous learning right, improve the mastering capabilities with the vivid contents, and enhance the learning efficiency with good interaction between teachers and students.However, we can′t ignore the deficiency of MOOC, such as it affects the quality of medical education for the fragmented knowledge, and it is unfavorable to the cultivation of students′ comprehensive qualities with the lack of school culture education.