Expression changes of Lumican and autophagy-related proteins in placenta of patients with preeclampsia

doi:10.3877/cma.j.issn.2095-655X.2021.01.009

Abstract

Abstract:

Objective

To investigate the expression changes of Lumican and autophagy-related proteins in placenta of patients with preeclampsia.

Methods

The placental specimens from 19 patients with preeclampsia and 21 healthy puerpera in the Obstetrics Department of People′s Hospital of Rizhao from January to December 2019 were collected. RT-qPCR and Western blotting were used to detect the expression of Lumican in human placenta. The expression levels of autophagy marker proteins microtubule-associated protein light chain 3 (LC3B) and Beclin1 in placental tissues were observed by immunohistochemical staining. The vitality of superoxide dismutase (SOD) and level of malondialdehyde (MDA) in placenta tissues were detected to analyse the level of oxidative stress.

Results

The expressions of Lumican mRNA (1.04±0.11) and protein (0.17±0.02) in PE group were lower than those in control group (2.81±0.11, 0.98±0.15, t=25.52, 12.07, all P<0.01). Immunohistochemical results showed that the expressions of Beclin1 and LC3B in PE group were lower than those in control group. The vitality of SOD in PE group [(201.31±26.11)U/mgprot] remarkablely decreased compared with that in control group [(241.65±25.28)U/mgprot] (t=3.50, P<0.01), while the level of MDA in PE group [(6.70±1.10)nmol/mgprot] remarkablely increased compared with that in control group [(5.10±1.05)nmol/mgprot] (t=3.33, P<0.01).

Conclusion

There is higher Lumican expression in human placental tissue, but the expression level of Lumican in placental tissue significantly decreases in PE, meanwhile it shows a higher level of oxidative stress, and decreased expression of autophagy related proteins.

Key words: Lumican, Pre-eclampsia, Autophagy, Oxidative stress

Huijie Gao, Zhongying Wang, Yulian Hu, Hua Pan, Chao Liu. Expression changes of Lumican and autophagy-related proteins in placenta of patients with preeclampsia[J]. Chinese Journal of Diagnostics(Electronic Edition), 2021, 09(01): 35-39.

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Figures/Tables 6

References 17

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项目	先兆子痫组(n＝19)	对照组(n＝21)	t值	P值
产妇年龄(岁)	30.05±2.74	30.33±3.45	0.28	>0.05
初潮年龄(岁)	14.05±0.71	14.05±0.74	0.02	>0.05
孕龄(周)	36.05±1.90	39.24±0.77	7.08	<0.01
胎儿体重(kg)	2.61±0.42	3.43±0.30	7.11	<0.01
收缩压(mmHg)	159.37±13.53	114.29±10.00	12.06	<0.01
舒张压(mmHg)	103.21±8.65	74.38±5.07	13.01	<0.01

项目	先兆子痫组(n＝19)	对照组(n＝21)	t值	P值
产妇年龄(岁)	30.05±2.74	30.33±3.45	0.28	>0.05
初潮年龄(岁)	14.05±0.71	14.05±0.74	0.02	>0.05
孕龄(周)	36.05±1.90	39.24±0.77	7.08	<0.01
胎儿体重(kg)	2.61±0.42	3.43±0.30	7.11	<0.01
收缩压(mmHg)	159.37±13.53	114.29±10.00	12.06	<0.01
舒张压(mmHg)	103.21±8.65	74.38±5.07	13.01	<0.01

基因	引物序列	PCR产物长度
β-肌动蛋白^a	正向：CATGTACGTTGCTATCCAGGC	250 bp
	反向：CTCCTTAATGTCACGCACGAT
Lumican	正向：CTTCAATCAGATAGCCAGACTGC	150 bp
	反向：AGCCAGTTCGTTGTGAGATAAAC

基因	引物序列	PCR产物长度
β-肌动蛋白^a	正向：CATGTACGTTGCTATCCAGGC	250 bp
	反向：CTCCTTAATGTCACGCACGAT
Lumican	正向：CTTCAATCAGATAGCCAGACTGC	150 bp
	反向：AGCCAGTTCGTTGTGAGATAAAC

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