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Chinese Journal of Diagnostics(Electronic Edition) ›› 2021, Vol. 09 ›› Issue (03): 187-191. doi: 10.3877/cma.j.issn.2095-655X.2021.03.011

• Clinical Study • Previous Articles     Next Articles

Diagnostic feature analysis of secondary agglutination of cross matching in children caused by polyagglutination of red blood cells

Fansheng Kong1,(), Wei Geng1   

  1. 1. Blood Type Reference Laboratory, The Blood Center of Jining, Jining 272113, China
  • Received:2020-10-09 Online:2021-08-26 Published:2021-09-03
  • Contact: Fansheng Kong

Abstract:

Objective

To investigate the characteristics and detection methods of secondary agglutination of cross matching in children with polyagglutination of red blood cells (RBCs).

Methods

The detection results of 8 child cases with cross matching secondary hemagglutination from January to December 2019 in Blood Group Reference Laboratory of Jining Blood Center were retrospectively analyzed. After ABO and Rh blood type identification, direct antiglobulin test (DAT), antibody screening and cross matching methods, false agglutination was excluded. Polyagglutinated RBCs were confirmed by 3 normal adults of homotypic blood serum, 3 normal adult AB serum and peanut agglutinin.

Results

In the 8 cases, the DAT of RBCs antibody screenings and the primary side of cross matching blood were negative. While the results from the secondary saline method and anti-human globulin method were positive. The RBCs of the children were agglutinated with AB serum and peanut agglutinin. All the elements showed an agglutination reaction, and the saline was used to eliminate false agglutination like rouleaux.

Conclusions

The polyagglutinated RBCs agglutinate with most adults sera, which lead to secondary positive in cross matching test. Confirming the polyagglutination of erythrocytes will help to identify the causes of cross matching difficulties and avoid delaying transfusion for child patients.

Key words: Polyagglutination of red blood cells, Peanut agglutinin, Cross matching, T/Tn poly agglutination antigen and gene

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