The value of proton magnetic resonance spectroscopy in assessing liver lipid and glucose metabolism alternations in rabbits with acute liver injury

doi:10.3877/cma.j.issn.2095-655X.2024.03.004

Abstract

Abstract:

Objective

To investigate the value of proton magnetic resonance spectroscopy (¹H-MRS) in assessing liver lipid and glucose metabolism alterations in a rabbit model of acute liver injury.

Methods

Twenty healthy adult male New Zealand white rabbits were randomly divided into a control group (n=10) and an experimental group (n=10). The control group received intraperitoneal injection of normal saline (1 ml/kg), while the experimental group received intraperitoneal injection of sodium selenite (1 ml/kg) to establish an acute liver injury model. After 48 hours, the 1.5 T magnetic resonance system was used to scan the live rabbit liver using a single-voxel point resolved spectroscopy sequence. LCModel software was employed to calculate the relative peaks of lipid, glycogen and glucose complex (Glyu), and choline-containing compounds (CCC) with water, and the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in rabbit serum were measured. Comparisons between the 2 groups were performed using t-tests. The rabbit liver specimens were stained with hematoxylin and eosin (HE) to observe pathological changes in liver tissues.

Results

Nineteen animals ultimately completed the experimental process, with 9 in the experimental group and 10 in the control group. Compared to the control group, the experimental group showed significantly increased levels of lipid 13/lipid 09 (5.21±1.83, 7.17±2.56) and lipid 13/(lipid 21+ lipid 23) (6.52±0.82, 7.35±2.33), while the levels of Glyu [(0.22±0.15)×10^-2, (0.15±0.06)×10^-2] and CCC [(0.09±0.03)×10^-2, (0.07±0.03)×10^-2] significantly decreased, all with statistically significant differences (t=-2.31, -2.88, 2.42, 2.12, all P<0.05). Serum AST [(433.79±140.19)U/L, (46.51±17.71)U/L] and ALT [(779.67±149.54)U/L, 69.44±20.79)U/L] levels in the experimental group and control group were also significantly elevated (t=5.46, 13.92, all P<0.05). The livers in the experimental group displayed pathological changes acute diffuse liver injury, such as destruction of the liver lobule structure and necrosis of liver cells.

Conclusions

Sodium selenite can effectively induced acute liver injury in the rabbit model. ¹H-MRS technology is proved to be a valuable tool for evaluating the metabolic of lipid and glucose associated with acute liver injury, offering a novel perspective for clinical non-invasive diagnostic strategies.

Key words: Chemical and drug induced liver injury, Magnetic resonance spectroscopy, Lipid, Glucose

Jitian Guan, Kezeng You, Yiqun Geng, Shuyi Sun, Lingfeng Lai, Zhiwei Shen, Xiaolei Zhang, Teng Zhou, Huaidong Huang, Lin Yang, Yan Cheng, Shuohua Wu, Zhihong Zhao, Caiyu Zhuang, Renhua Wu. The value of proton magnetic resonance spectroscopy in assessing liver lipid and glucose metabolism alternations in rabbits with acute liver injury[J]. Chinese Journal of Diagnostics(Electronic Edition), 2024, 12(03): 160-165.

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References 31

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