This consensus presents systematic guidelines for the standardized construction and management of gastrointestinal tumor biobanks, aiming to address the prominent issues of inconsistent sample quality and insufficient data traceability in current biobank practice. Given the high incidence of gastrointestinal tumors and the research on molecular mechanisms relying on high-quality biospecimens, this evidence-based consensus was developed by multidisciplinary experts, covering comprehensive standards for the entire process of sample collection, processing and storage. It defines differentiated processing strategies for surgical tissues, blood samples and other common biospecimens, and while clarifying collection timeframes, preservation conditions and data labeling requirements. Furthermore, it establishes a full-lifecycle management system covering sample information management, quality control, ethical compliance, and sample utilization/destruction protocols, with emphasis on the implementation of informed consent, data security protection, and compliance with genetic resource management regulations. This consensus provides normative guidance for standardized operation of gastrointestinal tumor biobanks, facilitates multicenter research collaboration and the clinical translation of precision medicine, and promotes the standardized development of tumor prevention and treatment research.