Influence of the polymorphism of glutathione S-transferases on the intelligence development of six-month-old infants

doi:10.3877/cma.j.issn.2095-655X.2016.03.013

Abstract

Abstract:

Objective

To explore the possible association between the polymorphism of glutathione S-transferases (GST) and the intelligence development of six-month-old infants.

Methods

DNA was extracted from the cord blood, and the GSTM1-02 genotypes were measured by multiple polymerase chain reaction (PCR), and the genotypes of GSTM3 (rs7483) and GSTT2(rs140195, rs2719) were measured by the SequenomMassArray system.Gesell development diagnostic scale was used to measure the development quotients (DQs) of 5 domains.

Results

The percentage of DQs less than 90 of the 5 domains was from 2.3% to 6.8%.The valid and invalid genotypes of GSTM1-02 was almost in the same percentage.The minor allele frequency of rs7483, rs140195 and rs2719 were 25.3%, 35.2% and 25.6%, respectively.Multivariate analysis showed that CC genotype indicated higher risk for lower DQs of adaptive, gross motor and fine motor behaviors compared to TT genotype with OR and 95% CI 10.51(1.31, 84.41), 4.44(1.18, 16.72)and 3.61(1.02, 12.74), respectively.

Conclusion

The polymorphism of rs7483 of GSTM3 may influence the intelligence development even when the infant is 6 months old.

Key words: Glutathione S-transferase, Polymorphism, Infant, Infant development

Xiao Jia, Qian Zhang, Zhongqing Sun, Meng Xie, Bingling Wang, Shutao Pang. Influence of the polymorphism of glutathione S-transferases on the intelligence development of six-month-old infants[J]. Chinese Journal of Diagnostics(Electronic Edition), 2016, 04(03): 198-202.

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Figures/Tables 5

References 19

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变量		例数(%)
前4个月的喂养方式		?
?	纯母乳喂养	253(45.3)
?	混合喂养	226(40.4)
?	纯人工喂养	80(14.3)
母亲的受教育程度小学及以下		?
母亲的受教育程度小学及以下		13 (2.3)
?	初中	162(29.0)
?	高中或中专	186(33.3)
?	大学专科、本科	189(33.8)
?	硕士及以上	9(1.6)
父亲的受教育程度		?
?	小学及以下	9(1.6)
?	初中	133(23.8)
?	高中或中专	211(37.8)
?	大学专科、本科	195(35.0)
?	硕士及以上	10(1.8)
家庭人均月收入		?
?	<2 000元	40 (7.1)
?	2 000～5 000元	397(71.0)
?	>5 000元	122(21.9)

变量		例数(%)
前4个月的喂养方式		?
?	纯母乳喂养	253(45.3)
?	混合喂养	226(40.4)
?	纯人工喂养	80(14.3)
母亲的受教育程度小学及以下		?
母亲的受教育程度小学及以下		13 (2.3)
?	初中	162(29.0)
?	高中或中专	186(33.3)
?	大学专科、本科	189(33.8)
?	硕士及以上	9(1.6)
父亲的受教育程度		?
?	小学及以下	9(1.6)
?	初中	133(23.8)
?	高中或中专	211(37.8)
?	大学专科、本科	195(35.0)
?	硕士及以上	10(1.8)
家庭人均月收入		?
?	<2 000元	40 (7.1)
?	2 000～5 000元	397(71.0)
?	>5 000元	122(21.9)

DQs		男性(n＝288)	女性(n＝271)	合计(n＝559)	Fisher检验	P值
适应性行为[M(min，max)]		98(66，115)	98(79，111)	98(66，115)	0.68	0.73
?	DQ<90	11(3.8)	8(3.0)	19(3.4)	?	?
?	110>DQ≥90	275(95.5)	262(96.7)	537(96.1)	?	?
?	DQ≥110	2(0.7)	1(0.4)	3(0.5)	?	?
大运动行为[M(min，max)]		98(77，110)	98(77，117)	98(77，117)	0.61	0.81
?	DQ<90	13(4.5)	10(3.7)	23(4.1)	?	?
?	110>DQ≥90	273(94.8)	260(95.9)	533(95.3)	?	?
?	DQ≥110	2(0.7)	1(0.4)	3(0.5)	?	?
精细动作行为[M(min，max)]		99(82，115)	98(73，117)	99(73，117)	0.32	0.92
?	DQ<90	16(5.6)	14(5.2)	30(5.4)	?	?
?	110>DQ≥90	269(93.4)	253(93.4)	522(93.4)	?	?
?	DQ≥110	3(1.0)	4(1.5)	7(1.3)	?	?
语言行为[M(min，max)]		101(76，119)	101(75，115)	101(75，119)	0.19	1.00
?	DQ<90	7(2.4)	6(2.2)	13(2.3)	?	?
?	110>DQ≥90	279(96.9)	263(97.0)	542(97.0)	?	?
?	DQ≥110	2(0.7)	2(0.7)	4(0.7)	?	?
个人社交行为[M(min，max)]		100(81，124)	100(77，108)	100(77，124)	3.80	0.10
?	DQ<90	15(5.2)	23(8.5)	38(6.8)	?	?
?	110>DQ≥90	271(94.1)	248(91.5)	519(92.8)	?	?
?	DQ≥110	2(0.7)	0(0.0)	2(0.4)	?	?

DQs		男性(n＝288)	女性(n＝271)	合计(n＝559)	Fisher检验	P值
适应性行为[M(min，max)]		98(66，115)	98(79，111)	98(66，115)	0.68	0.73
?	DQ<90	11(3.8)	8(3.0)	19(3.4)	?	?
?	110>DQ≥90	275(95.5)	262(96.7)	537(96.1)	?	?
?	DQ≥110	2(0.7)	1(0.4)	3(0.5)	?	?
大运动行为[M(min，max)]		98(77，110)	98(77，117)	98(77，117)	0.61	0.81
?	DQ<90	13(4.5)	10(3.7)	23(4.1)	?	?
?	110>DQ≥90	273(94.8)	260(95.9)	533(95.3)	?	?
?	DQ≥110	2(0.7)	1(0.4)	3(0.5)	?	?
精细动作行为[M(min，max)]		99(82，115)	98(73，117)	99(73，117)	0.32	0.92
?	DQ<90	16(5.6)	14(5.2)	30(5.4)	?	?
?	110>DQ≥90	269(93.4)	253(93.4)	522(93.4)	?	?
?	DQ≥110	3(1.0)	4(1.5)	7(1.3)	?	?
语言行为[M(min，max)]		101(76，119)	101(75，115)	101(75，119)	0.19	1.00
?	DQ<90	7(2.4)	6(2.2)	13(2.3)	?	?
?	110>DQ≥90	279(96.9)	263(97.0)	542(97.0)	?	?
?	DQ≥110	2(0.7)	2(0.7)	4(0.7)	?	?
个人社交行为[M(min，max)]		100(81，124)	100(77，108)	100(77，124)	3.80	0.10
?	DQ<90	15(5.2)	23(8.5)	38(6.8)	?	?
?	110>DQ≥90	271(94.1)	248(91.5)	519(92.8)	?	?
?	DQ≥110	2(0.7)	0(0.0)	2(0.4)	?	?

基因	SNP基因型	基因型	等位基因
GSTM1	GSTM1-02	?	－
	无效	309(55.3)	?
	有效	250(44.7)	?
GSTM3	rs7483	?	?
	CC	36(6.4)	C：283(25.3)
	TT	312(55.8)	T：835(74.7)
	CT	211(37.8)	?
GSTT2	rs140195	?	?
	AA	18(3.2)	A：394(35.2)
	GG	183(32.7)	G：724(64.8)
	GA	358(64.1)	?
	rs2719	?	?
	TT	19(3.4)	T：286(25.6)
	GG	292(52.3)	G：832(74.4)
	GT	248(44.3)	?

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