Osteopontin gene locus 708C/T polymorphism in children with Kawasaki disease

doi:10.3877/cma.j.issn.2095-655X.2020.02.009

Abstract

Abstract:

Objective

To investigate the polymorphism of locus 708C/T in Osteopontin (OPN) gene of children with Kawasaki disease (KD) and the correlation between the polymorphism and coronary artery lesion (CAL).

Methods

A total of 103 cases of patients with KD hospitalized in pediatrics department of the Third Xiangya Hospital of Central South University from October 2006 to March 2015 and 100 cases healthy children who underwent physical examination in the same period were selected as the study subjects. In the case-control study, polymorphism analysis of locus 708C/T in OPN gene of 203 cases were detected using PCR-RFLP method. According to the presence or absence of CAL, the KD group was divided into KD combined with CAL (n=43) and KD without CAL (n=60).

Results

In KD group, the 3 kinds of genotype distribution frequencies of OPN gene 708C/T site were CC (8.74%), CT (29.13%), TT (62.14%), and the allelic frequencies were C (23.30%) and T (76.70%), respectively. In normal control group, the 3 kinds of genotype distribution frequencies of OPN gene 708C/T site were CC (8.00%), CT (35.00%), TT (57.00%), and the allelic frequencies were C (25.50%) and T (74.50%). There were no significant differences between the two groups (χ²=0.79, 0.28, all P>0.05). The frequencies of three genotypes of OPN gene 708C/T site in KD with CAL group were CC (6.98%), CT (34.88%) and TT (58.14%), and the allelic frequencies were C (24.42%) and T (75.58%), respectively. The frequencies of OPN gene 708C/T site in KD without CAL group were CC (10.00%), CT (23.33%) and TT(66.67%), and the allelic frequencies were C (21.67%) and T (78.33%), respectively. There were no significant differences between the two groups (χ²=1.73, 0.22, all P>0.05).

Conclusion

No correlation is found between the polymorphism of locus 708C/T in OPN gene and the occurrence of KD and its complication CAL.

Key words: Mucocutaneous lymph node syndrome, Osteopontin, Polymorphism, single nucleotide, Coronary vessels

Pengzhu Li, Xia Zhan, Gui Xie, Xin Liu, Yujie Qian, Zhuoying Li, Lihua Huang, Zuocheng Yang. Osteopontin gene locus 708C/T polymorphism in children with Kawasaki disease[J]. Chinese Journal of Diagnostics(Electronic Edition), 2020, 08(02): 107-111.

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Figures/Tables 5

References 20

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组别		例数	基因型(%)			χ²值	P值
组别		例数	CT	TT	CC	χ²值	P值
对照组		?	?	?	?	0.19	0.91
?	实际频数	100	35.00	57.00	8.00	?	?
?	理论频数	100	38.00	55.50	6.50	?	?
KD组		?	?	?	?	1.40	0.50
?	实际频数	103	30.00	64.00	9.00	?	?
?	理论频数	103	33.82	60.59	5.59	?	?

组别		例数	基因型(%)			χ²值	P值
组别		例数	CT	TT	CC	χ²值	P值
对照组		?	?	?	?	0.19	0.91
?	实际频数	100	35.00	57.00	8.00	?	?
?	理论频数	100	38.00	55.50	6.50	?	?
KD组		?	?	?	?	1.40	0.50
?	实际频数	103	30.00	64.00	9.00	?	?
?	理论频数	103	33.82	60.59	5.59	?	?

组别	例数	基因型			等位基因
组别	例数	CT	TT	CC	T	C
对照组	100	35(35.00)	57(57.00)	8(8.00)	149(74.50)	51(25.50)
KD组	103	30(29.13)	64(62.14)	9(8.74)	158(76.70)	48(23.30)
χ²值	?	0.79			0.28
P值	?	>0.05			>0.05

组别	例数	基因型			等位基因
组别	例数	CT	TT	CC	T	C
对照组	100	35(35.00)	57(57.00)	8(8.00)	149(74.50)	51(25.50)
KD组	103	30(29.13)	64(62.14)	9(8.74)	158(76.70)	48(23.30)
χ²值	?	0.79			0.28
P值	?	>0.05			>0.05

组别	例数	基因型			等位基因
组别	例数	CT	TT	CC	T	C
CAL组	43	15(34.88)	25(58.14)	3(6.98)	65(75.58)	21(24.42)
无CAL组	60	14(23.33)	40(66.67)	6(10.00)	94(78.33)	26(21.67)
χ²值	?	1.73			0.22
P值	?	>0.05			>0.05

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