探讨通过16S核糖体RNA(16S rRNA )基因测序的方法用于临床菌株鉴定。

以细菌16S rRNA基因为靶序列，采用细菌的通用引物P1、P2进行PCR扩增，同时送到测序公司测序从而达到鉴定。

待测菌株及ATCC25923阳性对照通过PCR得到的扩增片段为1 465bp左右，待测菌株经测序比对鉴定为一株杰氏棒状杆菌，阳性质控ATCC25923鉴定为一株金黄的葡萄球菌，阴性对照未出现任何结果。

通过PCR检测细菌16srRNA基因可以应用于临床菌株的鉴定，并且适用一些难以鉴定的细菌。

To detect clinical strain by amplifying 16S ribosomal RNA(16S rRNA) gene with polymerase chain reaction (PCR) method.

16S rRNA gene was amplifyied by universal primers P1, P2 with polymerase chain reaction, and the polymerase chain reaction products were sequenced to achieve the DNA sequences.

PCR products were about 1 465bp, and the PCR sequences were blasted in NCBI web to achieve the proper strain name, with the use of this method, the unkown gram-positive bacilli strain was identified as corynebacterium jeikeium strain, ATCC25923 was identified as a strain of staphylococcus aureus and negative control was also specific.

16S rRNA gene detection can be used as strain identification, and especially for some less common clinical strains.