The expression of Bad mRNA in Hela cells infected by coxsackievirus B3

doi:10.3877/cma.j.issn.2095-655X.2014.04.006

Abstract

Abstract:

Objective

To investigate the significance of Bad mRNA induced by coxsackievirus B3 (CVB3).

Methods

After HeLa cells infected by CVB3, the cellular morphology at different times of infection was observed under the inverted microscope, and Bad mRNA expression was determined by RT-PCR.The difference of Bad mRNA expression was compared with t-test.

Results

The cellular morphology in control group was not obviously changed at different time points, but the cytopathic effect (CPE) in viral groups was significantly gradually changed at the infection time, which was most obvious at 24 h. At the meantime, the expression of Bad mRNA in control group was not significantly different at different infection time points(P>0.05), which was the same in the viral groups.But Bad mRNA expression in CVB3 group(0.61±0.06) was decreased compared with the control (0.76±0.06)(t=-3.445, P<0.05) at 24 h. However, there was no significance at other infection points such as 3h, 6h, 9h and 12h.

Conclusions

As the pro-apoptotic gene of Bad mRNA, plays an important role in the CVB3 infection which induces the apoptosis of Hela cells.

Key words: Enterovirus B, human, HeLa cells, Apoptosis

Lang Tian, Xin Li, Li Yang, Jia Chen, Zuocheng Yang. The expression of Bad mRNA in Hela cells infected by coxsackievirus B3[J]. Chinese Journal of Diagnostics(Electronic Edition), 2014, 02(04): 266-269.

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Figures/Tables 3

References 14

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组别	标本数	3h	6h	9h	12h	24h
对照组	4	0.53±0.05	0.62±0.03	0.63±0.18	0.67±0.09	0.76±0.06
病毒组	4	0.51±0.01	0.60±0.06	0.67±0.10	0.60±0.11	0.61±0.06
t值	?	－0.97	－0.43	0.39	－0.97	－3.45
P值	?	0.40	0.69	0.71	0.37	0.05

组别	标本数	3h	6h	9h	12h	24h
对照组	4	0.53±0.05	0.62±0.03	0.63±0.18	0.67±0.09	0.76±0.06
病毒组	4	0.51±0.01	0.60±0.06	0.67±0.10	0.60±0.11	0.61±0.06
t值	?	－0.97	－0.43	0.39	－0.97	－3.45
P值	?	0.40	0.69	0.71	0.37	0.05

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