The expression of Bad mRNA in Hela cells infected by coxsackievirus B3

doi:10.3877/cma.j.issn.2095-655X.2014.04.006

Abstract

Abstract:

Objective

To investigate the significance of Bad mRNA induced by coxsackievirus B3 (CVB3).

Methods

After HeLa cells infected by CVB3, the cellular morphology at different times of infection was observed under the inverted microscope, and Bad mRNA expression was determined by RT-PCR.The difference of Bad mRNA expression was compared with t-test.

Results

The cellular morphology in control group was not obviously changed at different time points, but the cytopathic effect (CPE) in viral groups was significantly gradually changed at the infection time, which was most obvious at 24 h. At the meantime, the expression of Bad mRNA in control group was not significantly different at different infection time points(P>0.05), which was the same in the viral groups.But Bad mRNA expression in CVB3 group(0.61±0.06) was decreased compared with the control (0.76±0.06)(t=-3.445, P<0.05) at 24 h. However, there was no significance at other infection points such as 3h, 6h, 9h and 12h.

Conclusions

As the pro-apoptotic gene of Bad mRNA, plays an important role in the CVB3 infection which induces the apoptosis of Hela cells.

Key words: Enterovirus B, human, HeLa cells, Apoptosis

Lang Tian, Xin Li, Li Yang, Jia Chen, Zuocheng Yang. The expression of Bad mRNA in Hela cells infected by coxsackievirus B3[J]. Chinese Journal of Diagnostics(Electronic Edition), 2014, 02(04): 266-269.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhzdxdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-655X.2014.04.006

https://zhzdxdzzz.cma-cmc.com.cn/EN/Y2014/V02/I04/266

Figures/Tables 3

References 14

[1]	杨作成．心肌炎的诊断与治疗[J]．实用儿科临床杂志，2010，25(10):782-784.
[2]	Valaperti A, Marty RR, Kania G，et al.CD11b＋ monoaytes abrogate Th17 CD4＋ T cell-mediated experimental autoimmune myocarditis[J]．J Immunol，2008，180(4):2686-2695.
[3]	Zorc-Pleskovic R, Alibegovi A, Zorc M，et al.Apoptosis of cardiomyocytes in myocarditis[J]．Folia Biol(Praha)，2006，52(1-2):6-9.
[4]	陈淳媛，孙跃女，杨作成，等．雷帕霉素对柯萨奇病毒B3诱导的大鼠心肌细胞mTOR和eIF-4E表达的调控作用[J]．中南大学学报(医学版)，2008，33(7):612-617.
[5]	Fuchs Y, Steller H．Programmed cell death in animal development and disease[J]．Cell，2011，147(4):742-758.
[6]	Debiasi RL, Edelstein CL, Sherry B，et al.Calpain inhibition protects against virus-induce myocardial injury[J]．J Virol，2001，75(1):351-361.
[7]	宋晓东，刘哲，辛颖，等．针对病毒性心肌炎的细胞凋亡研究[J]．中国现代医学杂志，2006，16(23):3549-3552.
[8]	杨志寅．现代医学科学发展中的缺憾与思考[J/CD]．中华诊断学电子杂志，2013，1(1):1-7.
[9]	Kawano H, Okada R, Kanano Y，et al.Apoptosis in acute and chronic myocarditis[J]．Jpn Heart J，1994，35(6):745-750.
[10]	田朗，朱咏贵，成亮，等．CVB3感染对HeLa细胞凋亡的影响[J]．湖南师范大学学报(医学版)，2013，10(3):4-7.
[11]	Youle RJ, Strasser A．The Bcl-2 ptotein family：opposing activities that mediate cell death[J]．Nat Rev Mol Cell Biol，2008，9(1):47-59.
[12]	Yoo NJ, Soung YH, Lee SH，et al.Mutational analysis of the BH3 domains of proapoptotic Bcl-2 family genes Bad，Bmf and Bcl-G in laryngeal squamous cell carcinomas[J]．Tumori，2007，93(2):195-197.
[13]	李欣，张娟，杨丽，等．LY294002和Rapamycin对柯萨奇病毒B3诱导的HeLa细胞Bim和Bax表达的调控作用[J]．基础医学与临床，2013，33(10):1038-1044.
[14]	Martin U, Jarasch N, Nestler M，et al.Antiviral effects of pancaspase inhibitors on the replication of coxsackievirus B3[J]．Apoptosis，2007，12(3):525-533.

组别	标本数	3h	6h	9h	12h	24h
对照组	4	0.53±0.05	0.62±0.03	0.63±0.18	0.67±0.09	0.76±0.06
病毒组	4	0.51±0.01	0.60±0.06	0.67±0.10	0.60±0.11	0.61±0.06
t值	?	－0.97	－0.43	0.39	－0.97	－3.45
P值	?	0.40	0.69	0.71	0.37	0.05

组别	标本数	3h	6h	9h	12h	24h
对照组	4	0.53±0.05	0.62±0.03	0.63±0.18	0.67±0.09	0.76±0.06
病毒组	4	0.51±0.01	0.60±0.06	0.67±0.10	0.60±0.11	0.61±0.06
t值	?	－0.97	－0.43	0.39	－0.97	－3.45
P值	?	0.40	0.69	0.71	0.37	0.05

[1]	Kang Li, Liang Ji, Wei Zhao, Lemin Lin. Dual role of autophagy in biological progression of breast cancer [J]. Chinese Journal of Breast Disease(Electronic Edition), 2023, 17(04): 195-202.
[2]	Yingying Kong, Lutao Xie, Xiaochi Lu, Jiefeng Xu, Guangju Zhou, Mao Zhang. Protective effect and mechanism of sodium butyrate on cardio-brain injury after cardiac arrest resuscitation in pigs [J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2023, 16(05): 355-362.
[3]	Xiaoyan Zhang, Dongqiong Xiao, Hu Gao, Lin Chen, Fajuan Tang, Xihong Li. The protective effect and mechanism of overexpression of transcription factor 12 on the cerebral cortex of rats with sepsis-associated encephalopathy [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 540-549.
[4]	Xingchen Liu, Juan Liu, Baobao Wei, Jie Liu, Hui Liu. Correlation analysis of abnormal expression of XIAP and XAF1 with ovarian cancer [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(04): 419-427.
[5]	Shuoru Liu, Gongwei Wang, Bin Zhang, Shuhao Li, Cheng Hu. Oncolytic virus M1 activates endoplasmic reticulum stress pathway to induce apoptosis of prostate cancer cells [J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(04): 388-393.
[6]	Hui Yu, Jing Wang, Dan Du, Fan Yang. Mechanism of miR-301a-3p in proliferation and apoptosis of human ovarian granulosa KGN cells [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(03): 137-143.
[7]	Chunwen Deng, Song Chen, Pei Zhong, Shiqiang Min, Jian Wan. LncRNA CRNDE regulates lipopolysaccharide-induced inflammatory response and apoptosis in human alveolar epithelial cells via miR-181a-5p/SOX6 axis [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(03): 129-136.
[8]	Shiyao Zhang, Yanyan Xu, Qi Zhang, Chunqiang Li, Zhicheng Zhao, Gang Liu. The m⁶A-binding protein YTHDC2 affects proliferation and apoptosis of colorectal cancer cells by regulating the p38MAPK signaling pathway [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2023, 12(02): 117-124.
[9]	Feng Ying, Jing Wang, Xueqing Liu, Xiao Li. The effects of aquaporin 1 on the proliferation, migration, and apoptosis of human corneal endothelial cells [J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2023, 13(03): 146-151.
[10]	Abudurxiti Adilai·, Ao Fei, Xiaowen Xing, Shengqiang Xie, Rui Zhang, Xiaojuan Lan, Gang Cheng. Comparison of injury features of three cell models simulating traumatic brain injury in vitro [J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2023, 09(02): 69-75.
[11]	Di Yu, Haibo Yu, Huancheng Wu, Yuming Li, Bin Su, Xin Chen. Effect of differential expression of hairy and enhancer of split homolog-1 on cholesterol-stimulated vascular endothelial cells [J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2023, 13(05): 264-270.
[12]	Shidong Deng, Lingzhi Liu, Dayong Guo, Chao Wang, Zhongxin Huang, Huihui Zhang. Effects of SNHG1 gene silencing on proliferation, apoptosis, migration, and ferroptosis of bladder cancer cells [J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(07): 804-811.
[13]	Minjie Zhang, Xiaoshan Zhang, Shasha Duan, Yilu Shi, Jie Zhao, Tianhao Bai, Yaxi Wang. Hydrogen for treatment of myocardial ischemia-reperfusion injury: mechanism and prospect [J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(06): 744-748.
[14]	Ruye Guo, Liming Meng, Nan Chen, Yuying Song, Haiyan Yin, Yan Guo. Research status on the neuroprotective efficacy and mechanism of Apelin/APJ system in Parkinson disease model [J]. Chinese Journal of Diagnostics(Electronic Edition), 2023, 11(04): 276-282.
[15]	Tian Qiu, Miaojuan Yang, Bo Hu, Yi Guo, Yitao He. Research progress on the mechanism of mild hypothermia therapy for cerebral infarction [J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2023, 17(05): 518-521.

Please choose a citation manager

Content to export