The relationship between APL subtypes and chromosome t(15; 17)or PML-RARa fusion gene

doi:10.3877/cma.j.issn.2095-655X.2015.01.004

Chinese Journal of Diagnostics(Electronic Edition) ›› 2015, Vol. 03 ›› Issue (01): 15-18. doi: 10.3877/cma.j.issn.2095-655X.2015.01.004

Special Issue: ；

• Clinical Research of Tumour • Previous Articles Next Articles

The relationship between APL subtypes and chromosome t(15; 17)or PML-RARa fusion gene

Bei Zhu¹, Yanchao Duan², Keqiang Wang¹^,^†()

^1. Department of Clinical Laboratory, Affiliated Hospital of Taishan Medical College, Taian 271000, China
^2. Department of Hematology, Affiliated Hospital of Taishan Medical College, Taian 271000, China

Received:2014-11-17 Online:2015-02-26 Published:2015-02-26
Contact: Keqiang Wang
About author:
Corresponding author: Wang Keqiang, Email: wkqsd@163.com

Abstract

Abstract:

Objective

To investigate the relationship between the subtypes of acute promyelocytic leukemia (APL) and expression of chromosome translocationt(15; 17)or fusion gene of PML-RARa.

Methods

Fifty-five APL patients were studied in the research.APL subtypes were determined according to FAB classification.Conventional karyotype analysis was performed to study chromosome translocation t(15; 17) and fluorescent in situ hybridization(FISH)was employed to detect PML-RARa fusion gene.

Results

APL patients were classified into three subtpyes: M_3a, M_3b and M_3c.The occurrence of t(15; 17) and PML-RARa fusion gene were 82.61% (19/23)and 95.65%(22/23)in M_3a, 82.35%(14/17)and 94.12%(16/17)in M_3b, 80.00%(12/15)and 100.0%(15/15)in M_3c respectively(χ²=0.046, P>0.05; χ² =0.844, P>0.05). The expression of t(15; 17) and PML-RARa fusion gene were 81.82%(45/55)and 96.36%(53/55) respectively (χ²=6.476, P<0.05).

Conclusions

There are no statistic differences between the the APL subtypes and expression of chromosome translocation t(15; 17)or PML-RARa fusion gene.FISH may be a more sensitive technique than conventional karyotype analysis.

Key words: Leukemia, promyelocytic, acute, In situ hybridization, fluorescent, PML-RARa fusion gene

Bei Zhu, Yanchao Duan, Keqiang Wang. The relationship between APL subtypes and chromosome t(15; 17)or PML-RARa fusion gene[J]. Chinese Journal of Diagnostics(Electronic Edition), 2015, 03(01): 15-18.

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References 18

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型别	例数	t(15；17)易位		PML-RARa
型别	例数	＋	－	＋	－
M_3a	23	19(82.61)	4(17.39)	22(95.65)	1(4.35)
M_3b	17	14(82.35)	3(17.65)	16(94.12)	1(5.88)
M_3c	15	12(80.00)	3(20.00)	15(100.00)	0(0.00)
χ²值	?	0.046		0.844
P值	?	0.997		0.656

型别	例数	t(15；17)易位		PML-RARa
型别	例数	＋	－	＋	－
M_3a	23	19(82.61)	4(17.39)	22(95.65)	1(4.35)
M_3b	17	14(82.35)	3(17.65)	16(94.12)	1(5.88)
M_3c	15	12(80.00)	3(20.00)	15(100.00)	0(0.00)
χ²值	?	0.046		0.844
P值	?	0.997		0.656

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