The relationship between aged-related change of regulatory T cells and incidence of lung cancer in the elderly

doi:10.3877/cma.j.issn.2095-655X.2015.01.003

Abstract

Abstract:

Objective

To study the change of age-related regulatory T cells and its relationship with the high incidence of lung cancer in the elderly patients.

Methods

To set up Lewis lung cancer model, thirty-six C57BL/6 mice were divided into six groups according to the different months of age.The change of CD4⁺ CD25⁺ Foxp3⁺ Treg cells in spleen was evaluated among the six groups by flow cytometry method.

Results

The number of CD4⁺ CD25⁺ Foxp3⁺ Treg cells was higher in tumor group (7.96±3.22) than that in healthy group(2.56±0.95) at the same age (CD4⁺ CD25⁺ Foxp3⁺ /CD4⁺ , t=4.33, P<0.05). Besides, in the healthy groups, there was statistical difference among the three groups (F=23.13, P<0.05). Accumulation of the CD4⁺ CD25⁺ Foxp3⁺ Treg cells were accompanied with age, the elderly mice contained a significantly larger population of CD4⁺ CD25⁺ Foxp3⁺ Treg cells in their spleen than those in younger counterparts, and the elderly tumor group had the highest expression(11.97±3.62).

Conclusion

The results suggest a close relationship between the age-related changes of treg cells aging and the incidence of lung cancer in the elderly.

Key words: Aging, Flymphocytes, requlatory, Lung neoplasms

Lijing Zhu, Zhuqiang Qiu, Panfei Hou. The relationship between aged-related change of regulatory T cells and incidence of lung cancer in the elderly[J]. Chinese Journal of Diagnostics(Electronic Edition), 2015, 03(01): 12-14.

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References 18

[1]	Campisi J,Yaswen P. Aging and cancer cell biology, 2009[J]. Aging Cell, 2009, 8(3): 221-225.
[2]	Irminger-Finger I. Science of cancer and aging[J]. J Clin Oncol, 2007, 25(14): 1844-1851.
[3]	杨志寅. 现代医学科学发展中的缺憾与思考[J/CD]. 中华诊断学电子杂志, 2013, 1(1): 1-7.
[4]	Bhardwaj N. Harnessing the immune system to treat cancer[J]. J Clin Invest, 2007, 117(5): 1130-1136.
[5]	Erfani N,Hamedi-Shahraki M,Rezaeifard S, et al.FoxP3＋ regulatory T cells in peripheral blood of patients with epithelial ovarian cancer[J]. Iran J Immunol, 2014, 11(2): 105-112.
[6]	Garg SK,Delaney C,Toubai T, et al.Aging is associated with increased regulatory T-cell function[J]. Aging Cell, 2014, 13(3): 441-448.
[7]	Batura-Gabryel H,Foremska-Iciek J. Lung cancer in the elderly-increasing epidemiological problem of 21st century[J]. Rocz Akad Med Bialymst, 2005, 50(suppl1): 152-155.
[8]	Oberle N,Eberhardt N,Falk CS, et al.Rapid, suppression of cytokine transcription in human CD4^＋CD25^＋ T cells by CD4^＋Foxp3＋ regulatory T cells：independence of IL-2 consumption, TGF-beta, and various inhibitors of TCR signaling[J]. J Immunol, 2007, 179(6): 3578-3587.
[9]	Provinciali M,Smorlesi A. Immunoprevention and immunotherapy of cancer in aging[J]. Cancer Immunol Immunother, 2005, 54(2): 93-106.
[10]	Surh CD,Sprent J. Homeostasis of naive and memory T cells[J]. Immunity, 2008, 29(6): 848-862.
[11]	Simpson RJ,Cosgrove C,Ingram LA, et al.Senescent T-lymphocytes are mobilised into the peripheral blood compartment in young and older humans after exhaustive exercise[J]. Brain Behav Immun, 2008, 22(4): 544-551.
[12]	Hartigan-O′ Connor DJ,Poon C,Sinclair E, et al.Human CD4^＋ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells[J]. J Immunol Methods, 2007, 319(1-2): 41-52.
[13]	Fehérvri Z,Sakaguchi S. Development and function of CD25^＋ CD4^＋ regulatory T cells[J]. Curr Opin Immunol, 2004, 16(2): 203-208.
[14]	Chen ZF,Xu Q,Ding JB, et al.CD4^＋CD25^＋Foxp3＋ Treg and TGF-beta play important roles in pathogenesis of Uygur cervical carcinoma[J]. Eur J Gynaecol Oncol, 2012, 33(5): 502-507.
[15]	Zheng Y,Josefowicz SZ,Kas A, et al.Genome-wide analysis of Foxp3 target genes in developing and mature regulatory T cells[J]. Nature, 2007, 445(7130): 936-940.
[16]	Sharma S,Dominguez AL,Lustgarten J. High accumulation of T regulatory cells prevents the activation of immune responses in aged animals[J]. J Immunol, 2006, 177(12): 8348-8355.
[17]	Moo-Young TA,Larson JW,Belt BA, et al.Tumor-derived TGF-beta mediates conversion of CD4^＋ Foxp3＋ regulatory T cells in a murine model of pancreas cancer[J]. J Immunother, 2009, 32(1): 12-21.
[18]	Gottenberg JE,Lavie F,Abbed K, et al.CD4 CD25 high regulatory T cells are not impaired in patients with primary Sjögren′s syndrome[J]. J Autoimmun, 2005, 24(3): 235-242.

组别	例数	青年	中年	老年	F值	P值
健康组	18	1.35±0.32	2.13±0.59	3.58±0.74	23.13	<0.05
肺癌组	18	3.05±0.61	5.55±0.71	11.97±3.62	33.89	<0.05
t值	?	2.56	3.06	6.74	?	?
P值	?	<0.05	<0.05	<0.05	?	?

组别	例数	青年	中年	老年	F值	P值
健康组	18	1.35±0.32	2.13±0.59	3.58±0.74	23.13	<0.05
肺癌组	18	3.05±0.61	5.55±0.71	11.97±3.62	33.89	<0.05
t值	?	2.56	3.06	6.74	?	?
P值	?	<0.05	<0.05	<0.05	?	?

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