Application of rapid immunofluorescence detection of IE antigen of human cytomegalovirus in screening congenital human cytomegalovirus infection

doi:10.3877/cma.j.issn.2095-655X.2019.04.009

Abstract

Abstract:

Objective

To explore the value of rapid immunofluorescence assay for human cytomegalovirus (HCMV) IE antigen in screening congenital HCMV infection.

Methods

From January to December 2017, saliva specimens and plantar dried blood smears from 352 newborns were collected from obstetrics department of Anhui Women and Children′s Health Care Hospital. Virus isolation and rapid immunofluorescence assay of HCMV IE antigen were used to detect saliva samples from newborns. HCMV-DNA was detected by real-time PCR in plantar dried blood smears. The authenticity of rapid immunofluorescence assay of HCMV IE antigen and real-time PCR for detection of congenital HCMV infection were compared.

Results

The results of virus isolation showed that 5 positive samples were isolated from 352 salivary specimens, the positive rate was 1.42%. Only 4 cases were positive detected by real-time PCR, and the positive rate was 1.14%. The results of rapid immunofluorescence assay of HCMV IE antigen were identical with those of virus isolation. The sensitivity and specificity of real-time PCR were 80% and 100%. The sensitivity and specificity of rapid immunofluorescence assay of HCMV IE antigen were 100% respectively. The sensitivity of rapid immunofluorescence assay of HCMV IE antigen was higher than that of real-time PCR assay, but there was no significant difference (Z=0.94, P=0.350).

Conclusion

Rapid immunofluorescence assay of HCMV IE antigen may be a more accurate method for early diagnosis of congenital HCMV infection.

Key words: Cytomegalovirus, Antigens, viral, Fluoroimmunoassay, Diagnosis, Congenital abnormalities

Longfeng Jiang, Yan Jin, Yan Liu. Application of rapid immunofluorescence detection of IE antigen of human cytomegalovirus in screening congenital human cytomegalovirus infection[J]. Chinese Journal of Diagnostics(Electronic Edition), 2019, 07(04): 259-264.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhzdxdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-655X.2019.04.009

https://zhzdxdzzz.cma-cmc.com.cn/EN/Y2019/V07/I04/259

Figures/Tables 4

References 19

[1]	Alarcon A, Martinez-Biarge M, Cabañas F, et al. A prognostic neonatal neuroimaging scale for symptomatic congenital cytomegalovirus infection[J]．Neonatology，2016，110(4):277-285.
[2]	Alarcon A, Martinez-Biarge M, Cabañas F，et al.Clinical，biochemical，and neuroimaging findings predict long-term neurodevelopmental outcome in symptomatic congenital cytomegalovirus infection[J]．J Pediatr，2013，163(3):828-834.
[3]	Swanson EC, Schleiss MR.Congenital cytomegalovirus infection: new prospects for prevention and therapy[J]．Pediatr Clin North Am，2013，60(2):335-349.
[4]	Zavattoni M, Lombardi G, Garofoli F，et al.Neonatal HCMV-related polymicrogyria in seroimmune women: what is the optimal pregnancy management[J]．J Clin Virol，2018(108):141-146.
[5]	Wang L, Xu X, Zhang H，et al.Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis[J]．Virol J，2015(12):60.
[6]	Balcarek KB, Warren W, Smith RJ，et al.Neonatal screening for congenital cytomegalovirus infection by detection of virus in saliva[J]．J Infect Dis，1993，167(6):1433-1436.
[7]	Fatima T, Siddiqui H, Ghildiyal S，et al.Cytomegalovirus infection in pregnant women and its association with bad obstetric outcomes in Northern India[J]．Microb Pathog，2017(113):282-285.
[8]	Giannattasio A, Di Costanzo P, De Matteis A，et al.Outcomes of congenital cytomegalovirus disease following maternal primary and non-primary infection[J]．J Clin Virol，2017(96):32-36.
[9]	Giannattasio A, Bruzzese D, Di Costanzo P，et al.Neuroimaging profiles and neurodevelopmental outcome in infants with congenital cytomegalovirus infection[J]．Pediatr Infect Dis J，2018，37(10):1028-1033.
[10]	Dobbie AM.Evaluation and management of cytomegalovirus-associated congenital hearing loss[J]．Curr Opin Otolaryngol Head Neck Surg，2017，25(5):390-395.
[11]	Fletcher KT, Horrell EMW, Ayugi J，et al.The natural history and rehabilitative outcomes of hearing loss in congenital cytomegalovirus：a systematic review[J]．Otol Neurotol，2018，39(7):854-864.
[12]	冯绵烨，娄燕．病毒性脑炎的诊治研究进展[J/CD]．中华诊断学电子杂志，2019，7(1):66-70.
[13]	Lanzieri TM, Dollard SC, Bialek SR，et al.Systematic review of the birth prevalence of congenital cytomegalovirus infection in developing countries[J]．Int J Infect Dis，2014(22):44-48.
[14]	Kenneson A, Cannon MJ.Review and meta-analysis of the epidemiology of congenital cytomegalovirus (CMV) infection[J]．Rev Med Virol，2007，17 (4):253-276.
[15]	Dollard SC, Grosse SD, Ross DS.New estimates of the prevalence of neurological and sensory sequelae and mortality associated with congenital cytomegalovirus infection[J]．Rev Med Virol，2007，17 (5):355-363.
[16]	Soroceanu L, Matlaf L, Khan S，et al.Cytomegalovirus immediate-early proteins promote stemness properties in glioblastoma[J]．Cancer Res，2015，75(15):3065-3076.
[17]	赵杨，陈敦金，闻良珍．人巨细胞病毒基因时序表达对感染结局的影响[J]．中华微生物学和免疫学杂志，2004 (10):80-84.
[18]	Liu X, Yuan J, Wu AW, et al. Phorbol ester-induced human cytomegalovirus major immediate-early (MIE) enhancer activation through PKC-delta，CREB，and NF-kappaB desilences MIE gene expression in quiescently infected human pluripotent NTera2 cells[J]．J Virol，2010，84(17):8495-8508.
[19]	Biolatti M, Dell′Oste V, Scutera S，et al.The viral tegument protein pp65 impairs transcriptional upregulation of IL-1β by human cytomegalovirus through inhibition of NF-kB activity[J]．Viruses，2018，10(10):567.

检测方法	敏感度(%)	特异度(%)	假阴性(例)
荧光定量PCR检测法	80	100	1
HCMV IE抗原免疫荧光检测法	100	100	0
病毒分离培养法	100	100	0

检测方法	敏感度(%)	特异度(%)	假阴性(例)
荧光定量PCR检测法	80	100	1
HCMV IE抗原免疫荧光检测法	100	100	0
病毒分离培养法	100	100	0

Please choose a citation manager

Content to export