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中华诊断学电子杂志 ›› 2016, Vol. 04 ›› Issue (04) : 279 -283. doi: 10.3877/cma.j.issn.2095-655X.2016.04.017

所属专题: 文献

基础研究

大鼠侧脑室注射Apelin-13对急性痛的调节作用及机制
徐震1, 王庆尧1, 王贤斌1, 武菲1,()   
  1. 1. 272067 济宁医学院神经生物学研究所
  • 收稿日期:2016-06-13 出版日期:2016-11-26
  • 通信作者: 武菲
  • 基金资助:
    2016年地方高校国家级大学生创新创业训练计划项目(201610443048); 济宁医学院青年基金(JYQ14KJ19); 2015年度济宁市医药卫生计划项目(79); 济宁医学院大学生创新训练计划项目(CX2015005)

The modulation and mechanism of Apelin-13 in an acute pain model

Zhen Xu1, Qingyao Wang1, Xianbin Wang1, Fei Wu1,()   

  1. 1. Institute of Neurobiology, Jining Medical University, Jining 272067, China
  • Received:2016-06-13 Published:2016-11-26
  • Corresponding author: Fei Wu
  • About author:
    Corresponding author: Wu Fei, Email:
引用本文:

徐震, 王庆尧, 王贤斌, 武菲. 大鼠侧脑室注射Apelin-13对急性痛的调节作用及机制[J]. 中华诊断学电子杂志, 2016, 04(04): 279-283.

Zhen Xu, Qingyao Wang, Xianbin Wang, Fei Wu. The modulation and mechanism of Apelin-13 in an acute pain model[J]. Chinese Journal of Diagnostics(Electronic Edition), 2016, 04(04): 279-283.

目的

探讨Apelin-13对大鼠急性痛的调制作用及可能机制。

方法

简单随机抽样将50只大鼠分为生理盐水组、Apelin-13 0.05μg/g组、Apelin-13 0.1μg/g组、Apelin-13 0.5μg/g组及吗啡组(阳性对照组),每组10只,采用辐射热甩尾法连续监测60 min内大鼠痛阈的变化;同样采用简单随机抽样将40只大鼠分为生理盐水组、Apelin-13 0.5 μg/g组、酪蛋白激酶2(CK2)抑制剂四溴苯三唑(TBB)组及Apelin-13+TBB组,每组10只,监测其痛阈的变化。Western Blot法检测海马内CK2表达量及N-甲基-D-天冬氨酸(NMDA)受体NR2B亚单位(P-NR2B S1480)磷酸化水平,研究Apelin-13对急性痛调制作用可能的机制。

结果

Apelin-13可显著升高急性痛痛阈,0.5 μg/g组给药10 min时甩尾潜伏期变化率(TWL%)达峰值[(50.03±2.71)%],与生理盐水组比较差异有统计学意义(q=18.054,P=0.003),并且此效应呈剂量和时间依赖(FGroup=47.729,P=0.000;FTime=205.301,P=0.000)。同时给予CK2抑制剂TBB10 min时TWL%降至(17.75±1.67)%,低于Apelin-13组,差异有统计学意义(q=6.976,P=0.005)。Western Blot结果显示TBB可明显减弱Apelin-13的作用,CK2α表达量、NR2B亚单位磷酸化水平均下降[(26.92±4.38)%,(39.90±7.40)%],与Apelin-13组[(41.60±6.65)%,(70.83±3.52)%]比较差异有统计学意义(q=5.246,P=0.010;q=9.757,P=0.010)。

结论

Apelin-13可能通过CK2促进NMDA受体NR2B亚单位磷酸化,进而在热甩尾急性痛模型中起镇痛作用。

Objective

To explore the modulation and mechanism of Apelin-13 in an acute pain model.

Methods

The pain thresholds were continuously monitored in 60 min after intracerebroventricular (ICV) administration of Apelin-13 (0.05, 0.1 and 0.5 μg/g) and casein kinase 2(CK2) inhibitor 4, 5, 6, 7-tetrabromoben: zotriazole (TBB) by radiant heat tail-flick test.Then the expression of CK2 and phosphorylation of NR2B S1480 in hippocampus were detected by Western Blot.

Results

ICV injection of Apelin-13 induced a dose and time-dependent antinociceptive effect.This effect was signicantly antagonized by CK2 inhibitor TBB.Western Blot showed that Apelin-13 could significantly promote the expression of CK2 and the phosphorylation of NR2B S1480, which decreased obviously by TBB.

Conclusion

Apelin-13 plays an antinociceptive role in the acute pain model of radiant heat tail-flick test via modulating CK2 expression and the phosphorylation of NR2B S1480.

表1 大鼠侧脑室注射Apelin-13及生理盐水组、吗啡组甩尾潜伏期变化率的比较(每组n=10;%,±s)
表2 大鼠侧脑室注射Apelin-13及TBB后甩尾潜伏期变化率的比较(每组n=10;%,±s)
图1 大鼠侧脑室注射Apelin-13后酪蛋白激酶2表达水平、NR2B亚单位磷酸化水平
表3 大鼠侧脑室注射Apelin-13后CK2表达水平、NR2B磷酸化水平的比较(每组n=10;%,±s)
图2 大鼠侧脑室注射Apelin-13及TBB后酪蛋白激酶2表达水平、NR2B亚单位磷酸化水平
表4 大鼠侧脑室注射Apelin-13及TBB后CK2α表达水平、NR2B亚单位磷酸化水平的比较(%,±s)
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