The application value of contrast enhanced ultrasound for detection of renal microvascular blood perfusion in diabetic nephropathies rabbits

doi:10.3877/cma.j.issn.2095-655X.2020.01.002

Abstract

Abstract:

Objective

To explore the value of contrast enhanced ultrasound (CEUS) for detection of renal microvascular blood perfusion in diabetic nephropathies (DN) rabbits.

Methods

Twenty rabbits were randomly divided into control group (n=10) and DN model building group (n=10) according to the random number table method. After successful modeling, there were 6 rabbits in DN group and 9 rabbits in control group. Real-time and low acoustic power imaging of the renal cortex was performed using CEUS with SonoVue to observe renal microcirculation. The time intensity curve (TIC) was used to obtain quantitative parameters including time to peak (TTP), peak intensity (PI), mean transit time (MTT), area under the curve (AUC) and so on. Renal function and morphology were also compared. Independent t-test was used to analyze measurement data.

Results

TIC revealed the TTP[(31.91±6.21)s, (22.30±8.05)s] and MTT[(58.68±9.82)s, (53.37±4.47)s] were longer in DN group than those in control group, with significant differences (t=6.66, 3.80, all P<0.05). Meanwhile DN group had a significantly low PI compared with control group [(29.31±7.73)dB , (34.45±5.46)dB, t=4.86, P<0.05]. However, no statistical significant differences were found in the change of other quantitative parameters including AUC [(2 442.65±725.47)dB/s, (2 255.31±209.09)dB/s, t=0.38, P>0.05], slope coefficient during wash-in (0.41±0.08, 0.37±0.06, t=1.26, P>0.05), slope coefficient during wash-out (0.02±0.01, 0.02±0.01, t=0.26, P>0.05) in both DN and control group.

Conclusions

CEUS may be useful for the early diagnosis of DN. TTP, MTT and PI may be useful quantitative parameters.

Key words: Diabetic nephropathies, Contrast enhanced ultrasound, Rabbit, Quantitative analysis

Qun Wang, Jia Li, Heng Miao. The application value of contrast enhanced ultrasound for detection of renal microvascular blood perfusion in diabetic nephropathies rabbits[J]. Chinese Journal of Diagnostics(Electronic Edition), 2020, 08(01): 9-13.

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References 21

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组别	例数(只)	肾脏长径(cm)	肾脏厚径(cm)	皮质厚度(cm)	PSV(cm/s)	RI
对照组	9	3.41±0.56	1.75±0.31	0.52±0.37	46.52±11.21	0.48±0.11
DN组	6	3.38±0.42	1.77±0.34	0.53±0.24	47.14±10.56	0.50±0.20
t值		1.09	0.70	1.10	0.44	0.60
P值		0.45	0.52	0.43	0.61	0.53

组别	例数(只)	肾脏长径(cm)	肾脏厚径(cm)	皮质厚度(cm)	PSV(cm/s)	RI
对照组	9	3.41±0.56	1.75±0.31	0.52±0.37	46.52±11.21	0.48±0.11
DN组	6	3.38±0.42	1.77±0.34	0.53±0.24	47.14±10.56	0.50±0.20
t值		1.09	0.70	1.10	0.44	0.60
P值		0.45	0.52	0.43	0.61	0.53

组别	例数(只)	Scr(μmol/L)	BUN(mmol/L)
对照组	9	68.53±12.52	7.35±0.51
DN组	6	71.51±10.82	7.46±0.76
t值		1.84	1.41
P值		0.11	0.24

组别	例数(只)	Scr(μmol/L)	BUN(mmol/L)
对照组	9	68.53±12.52	7.35±0.51
DN组	6	71.51±10.82	7.46±0.76
t值		1.84	1.41
P值		0.11	0.24

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