The modulation and mechanism of Apelin-13 in an acute pain model

doi:10.3877/cma.j.issn.2095-655X.2016.04.017

Abstract

Abstract:

Objective

To explore the modulation and mechanism of Apelin-13 in an acute pain model.

Methods

The pain thresholds were continuously monitored in 60 min after intracerebroventricular (ICV) administration of Apelin-13 (0.05, 0.1 and 0.5 μg/g) and casein kinase 2(CK2) inhibitor 4, 5, 6, 7-tetrabromoben: zotriazole (TBB) by radiant heat tail-flick test.Then the expression of CK2 and phosphorylation of NR2B S1480 in hippocampus were detected by Western Blot.

Results

ICV injection of Apelin-13 induced a dose and time-dependent antinociceptive effect.This effect was signicantly antagonized by CK2 inhibitor TBB.Western Blot showed that Apelin-13 could significantly promote the expression of CK2 and the phosphorylation of NR2B S1480, which decreased obviously by TBB.

Conclusion

Apelin-13 plays an antinociceptive role in the acute pain model of radiant heat tail-flick test via modulating CK2 expression and the phosphorylation of NR2B S1480.

Key words: Apelin, Acute pain, Analgesia, Casein kinase Ⅱ, NR2B

Zhen Xu, Qingyao Wang, Xianbin Wang, Fei Wu. The modulation and mechanism of Apelin-13 in an acute pain model[J]. Chinese Journal of Diagnostics(Electronic Edition), 2016, 04(04): 279-283.

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Figures/Tables 6

References 19

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组别	0 min	5 min	10 min	20 min	30 min	40 min	50 min	60 min
生理盐水组	－0.80±0.81	－3.28±1.04	1.39±2.68	3.30±0.27	3.45±2.52	2.49±2.11	4.14±0.17	2.95±1.78
Apelin 0.05组	－1.65±0.56	9.24±2.78	17.31±2.00	14.93±1.63	5.41±1.75	3.97±1.08	0.33±1.20	1.07±0.58
Apelin 0.1组	－0.23±1.75	19.79±1.25	33.66±3.29	23.12±1.54	17.36±3.04	9.67±1.16	3.89±0.19	3.47±0.32
Apelin 0.5组	3.03±0.05	26.29±6.39	50.03±2.71	40.27±1.49	25.35±2.67	11.54±2.13	4.72±2.25	3.22±0.97
吗啡组	0.05±1.57	60.05±12.71	69.92±6.23	48.74±3.27	30.26±10.62	19.46±2.86	17.42±3.58	14.72±1.44
F值	4.813	26.701	59.700	90.150	10.134	23.313	22.239	25.200
P值	0.058	0.014	0.010	0.001	0.013	0.002	0.020	0.040

组别	0 min	5 min	10 min	20 min	30 min	40 min	50 min	60 min
生理盐水组	－0.80±0.81	－3.28±1.04	1.39±2.68	3.30±0.27	3.45±2.52	2.49±2.11	4.14±0.17	2.95±1.78
Apelin 0.05组	－1.65±0.56	9.24±2.78	17.31±2.00	14.93±1.63	5.41±1.75	3.97±1.08	0.33±1.20	1.07±0.58
Apelin 0.1组	－0.23±1.75	19.79±1.25	33.66±3.29	23.12±1.54	17.36±3.04	9.67±1.16	3.89±0.19	3.47±0.32
Apelin 0.5组	3.03±0.05	26.29±6.39	50.03±2.71	40.27±1.49	25.35±2.67	11.54±2.13	4.72±2.25	3.22±0.97
吗啡组	0.05±1.57	60.05±12.71	69.92±6.23	48.74±3.27	30.26±10.62	19.46±2.86	17.42±3.58	14.72±1.44
F值	4.813	26.701	59.700	90.150	10.134	23.313	22.239	25.200
P值	0.058	0.014	0.010	0.001	0.013	0.002	0.020	0.040

组别	0 min	5 min	10 min	20 min	30 min	40 min	50 min	60 min
生理盐水组	－0.83±0.78	－3.28±1.04	1.39±2.68	3.30±0.27	3.45±2.52	2.49±2.09	4.14±0.17	2.95±1.60
Apelin组	1.82±2.08	20.78±7.58	55.19±9.14	37.20±5.42	19.38±10.51	7.69±6.83	1.54±5.74	1.79±2.56
TBB组	－0.71±0.62	－2.32±3.27	－5.99±3.20	－4.71±1.24	－4.90±0.08	－3.20±10.13	－2.03±0.24	－2.43±0.80
Apelin ＋TBB组	－0.95±0.23	9.22±0.72	17.75±1.67	11.76±2.57	8.38±1.20	6.76±0.86	6.03±0.06	3.33±1.83
F值	2.264	14.432	56.422	70.328	5.586	2.788	1.866	3.433
P值	0.199	0.007	0.002	0.000	0.047	0.150	0.253	0.108

组别	0 min	5 min	10 min	20 min	30 min	40 min	50 min	60 min
生理盐水组	－0.83±0.78	－3.28±1.04	1.39±2.68	3.30±0.27	3.45±2.52	2.49±2.09	4.14±0.17	2.95±1.60
Apelin组	1.82±2.08	20.78±7.58	55.19±9.14	37.20±5.42	19.38±10.51	7.69±6.83	1.54±5.74	1.79±2.56
TBB组	－0.71±0.62	－2.32±3.27	－5.99±3.20	－4.71±1.24	－4.90±0.08	－3.20±10.13	－2.03±0.24	－2.43±0.80
Apelin ＋TBB组	－0.95±0.23	9.22±0.72	17.75±1.67	11.76±2.57	8.38±1.20	6.76±0.86	6.03±0.06	3.33±1.83
F值	2.264	14.432	56.422	70.328	5.586	2.788	1.866	3.433
P值	0.199	0.007	0.002	0.000	0.047	0.150	0.253	0.108

组别	CK2α	CK2β	p-NR2B S1480
生理盐水组	14.79±2.77	9.39±0.75	10.43±3.78
Apelin 0.05组	35.15±1.76	17.53±2.06	40.01±3.08
Apelin 0.1组	63.39±3.46	35.96±4.52	64.99±4.61
Apelin 0.5组	82.28±3.42	51.13±2.15	75.12±6.01
F值	307.851	141.065	121.129
P值	0.000	0.000	0.002

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